中医康复2025，Vol.2Issue(11)：1-5,5.DOI:10.19787/j.issn.2097-3128.2025.11.001
基于CircFOXO3介导软骨细胞糖酵解探讨乌头汤改善骨关节炎软骨退变的作用
Study on the Effect of Wutou Decoction on Improving Cartilage Degeneration of Osteoarthritis Based on CircFOXO3-Mediated Chondrocyte Glycolysis
摘要
Abstract
Objective:To explore the effect and mechanism of auriculotherapy in modulating CircFOXO3 to improve glycolysis in chondrocyte inflammation induced by interleukin-1β(IL-1β),and to provide experimental basis for the clinical application of auricu-lotherapy.Methods:20 3-week-old SPF C57BL/6 male mice were extracted from the cartilage of both knee joints for the isolation and culture of chondrocytes in vitro,and the chondrocyte model was induced by the action of 10 ng/mL IL-1β for 24 h in vitro,and CircFOXO3 overexpression cell line was constructed;chondrocytes were randomly divided into a blank group,an IL-1β group,an aconite soup group(10 ng/mL IL-1β+10%Wutou decoction containing serum),oe-CircFOXO3 group and oe-CircFOXO3+Wutou decoction group.The relative expression level of CircFOXO3 in chondrocytes of each group was detected by Real-time PCR.West-ern blot detected the protein content of GLUT1,PKM2,HK2 and LDHA in chondrocytes of each group.Results:CircFOXO3 ex-pression was significantly reduced in the IL-1β-induced chondrocyte model(P<0.05),and the protein expression of GLUT1,PKM2,HK2 and LDHA was enhanced in the IL-1β group compared with the blank group(P<0.05);the above protein levels were decreased in the oe-CircFOXO3+Wutou decoction group compared with the oe-CircFOXO3 group(P<0.05),and the protein levels of the above mentioned proteins were decreased in the oe-CircFOXO3+Wutou decoction group compared with the IL-1β group,Cir-cFOXO3 expression was enhanced in the oe-CircFOXO3 group(P<0.05).Conclusion:Wutou Decoction can inhibit osteoarthritic chondrocyte glycolysis and improve cartilage degeneration by regulating CircFOXO3.关键词
骨关节炎/乌头汤/CircFOXO3/糖酵解/软骨细胞Key words
osteoarthritis/Wutou decoction/CircFOXO3/glycolysis/chondrocytes分类
医药卫生引用本文复制引用
陈悦,蒋镔烨,庄莹莹,林艳铭,兰书洁,李宇涛..基于CircFOXO3介导软骨细胞糖酵解探讨乌头汤改善骨关节炎软骨退变的作用[J].中医康复,2025,2(11):1-5,5.基金项目
福建省自然科学基金项目(2020J01736) （2020J01736）
