长春中医药大学学报2025，Vol.41Issue(10)：1101-1106,6.DOI:10.13463/j.cnki.cczyy.2025.10.010
基于PI3K/AKT信号通路探讨木香烃内酯对卵巢癌细胞的影响
Exploration of the effect of costunolide on ovarian cancer cells based on the phosphatidylinositol 3-kinase/protein kinase B signaling pathway
摘要
Abstract
Objective To investigate the effects of costunolide on the proliferation,migration,and invasion of ovarian cancer A2780 and SKOV3 cells based on the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signaling pathway.Methods Ovarian cancer A2780 and SKOV3 cells were used as the research objects,and the blank control group,the low,medium,and high concentration groups of costunolide,and the positive control group(PI3K inhibitor group)were set up respectively.CCK-8 assay was used to detect the effect of costunolide on the proliferation of ovarian cancer A2780 and SKOV3 cells;Scratch assay was used to detect the effect of costunolide on the migration of ovarian cancer A2780 and SKOV3 cells;Transwell assay was used to examine the effect of costunolide on the invasion of ovarian cancer A2780 and SKOV3 cells.The expression levels of PI3K/AKT signaling pathway-related proteins p-PI3K,PI3K,p-AKT and AKT were detected by Western Blot.Results Compared with the blank control group,the costunolide treatment group showed inhibitory effects on the proliferation,migration,and invasion of ovarian cancer A2780 and SKOV3 cells in a concentration-dependent manner(P<0.05).Western blotting experiment results showed that the protein expression levels of p-PI3K/PI3K and p-AKT/AKT in the costunolide treatment group were lower than those in the blank control group(P<0.05).There were no statistically significant differences in p-PI3K/PI3K and p-AKT/AKT in the high concentration group of costunolide compared with the positive control group(PI3K inhibitor group)(P>0.05).Conclusion Costunolide may inhibit the proliferation,migration,and invasion of ovarian cancer cells by suppressing the PI3K/AKT signaling pathway.关键词
木香烃内酯/卵巢癌细胞/PI3K/AKT信号通路Key words
costunolide/ovarian cancer cells/phosphatidylinositol 3-kinase/protein kinase B signaling pathway分类
医药卫生引用本文复制引用
劳楚思,刘博娅,索玉平..基于PI3K/AKT信号通路探讨木香烃内酯对卵巢癌细胞的影响[J].长春中医药大学学报,2025,41(10):1101-1106,6.基金项目
国家自然科学基金项目(61975105,61775128) （61975105,61775128）
