大豆科学2025，Vol.44Issue(5)：45-54,10.DOI:10.11861/j.issn.1000-9841.2025.05.0045
大豆盐胁迫和干旱胁迫响应EXP基因鉴定及GmEXLB2克隆和诱导表达分析
Identification of Salt and Drought Stress-Responsive EXP Genes and Cloning and Induced Expression Analysis of GmEXLB2 in Soybean
摘要
Abstract
Soybean is an important economic crop for both oil and grain,and its yield is often severely affected by abiotic stresses such as drought and high salinity.Expansins(EXPs)are a class of proteins that play crucial roles in plants by inducing cell wall loosening.Through the regulation of plant cell walls,these proteins play significant roles in abiotic stress responses.This study aims to identify EXPs in soybean that respond to both salt and drought stresses.Based on transcriptome data from previous salt and drought stress treatments,a systematic analysis of EXP genes was conducted using methods such as Venn diagrams,bioinformatics analysis,real-time quantitative PCR,and gene cloning.The research findings indicated that a total of 49 GmEXPs respond to salt stress,while 21 GmEXPs respond to drought stress,with 15 GmEXPs co-regulated by both salt and drought stresses.Further analysis revealed that the promoter regions of these 15 GmEXPs contain multiple cis-acting elements related to plant stress responses and phytohormone responses.Additionally,this study cloned a gene,GmEXLB2,which was significantly induced by both drought and salt stresses,from the roots of the soybean variety Qihuang 34.This gene encoded an expansin-like B protein.The amino acid sequence of the GmEXLB2 protein exhibited high homology with that of the wild soybean expansin.This protein contained a conserved expansin domain,and its secondary structure was primarily composed of alpha helices and random coils.The expression level of the GmEXLB2 gene was the highest in the hypocotyl,and treatments with salt stress and drought stress significantly induce its expression in the roots.Additionally,the expression of the GmEXLB2 gene was regulated by plant hormones,with treatments of ACC,GA3,and ABA all leading to increased expression of GmEXLB2.The results of this study indicate that the GmEXLB2 gene plays a significant role in the plant's response to salt and drought stress.This gene may participate in soybean's response to these stresses through plant hormone signaling.This research provides new insights into the mechanisms by which soybean EXLB genes respond to adverse conditions and offers a theoretical foundation for further studies on the drought resistance and salt tolerance functions of the GmEXLB2 gene.关键词
大豆/盐胁迫/干旱胁迫/扩展蛋白/GmEXLB2/诱导表达分析Key words
soybean/salt stress/drought stress/expansins/GmEXLB2/inducible expression analysis引用本文复制引用
郑荣繁,于本帅,王丽丽,徐冉,王玉斌..大豆盐胁迫和干旱胁迫响应EXP基因鉴定及GmEXLB2克隆和诱导表达分析[J].大豆科学,2025,44(5):45-54,10.基金项目
山东省农业良种工程项目(2023LZG008) （2023LZG008）
泰山产业领军人才工程(tscx202211138) （tscx202211138）
沂蒙丘陵生态区粮烟双促双优模式的构建与应用项目(合同号202302) （合同号202302）
财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-04-CES12) （CARS-04-CES12）
济南市高层次人才专项(202228094). （202228094）
