大连工业大学学报2025，Vol.44Issue(5)：332-339,8.DOI:10.19670/j.cnki.dlgydxxb.2025.0504
CdnR2对Paraburkholderia caffeinilytica CF1咖啡因降解基因簇的转录调控
The transcriptional regulation of the caffeine degradation gene cluster by CdnR2 in Paraburkholderia caffeinilytica CF1
摘要
Abstract
A caffeine degrading gene cluster was discovered in the genome of a highly efficient caffeine degrading bacterium Paraburkholderia caffeinelytica CF1 isolated early in the laboratory.A transcription regulatory factor cdnR2 belonging to the LysR family was found on the gene cluster.The regulatory function of transcription regulatory factor cdnR2 was analyzed in the caffeine degradation of strain CF1.The regulatory function of cdnR2 caffeine degradation in strain CF1 was analyzed by constructing mutants,qRT-PCR and EMSA.The results indicated that CdnR2 inhibited the transcription of cdnR1 by directly binding to PR1 and PK,thereby promoting the transcription of cdnI,cdnJ and cdnK,and indirectly regulating the transcription of cdnH.In addition,caffeine and its degradation intermediates enhance the binding affinity of CdnR2 to PR1 and PK.关键词
Paraburkholderia caffeinilytica/咖啡因/降解/LysR家族转录调控因子Key words
Paraburkholderia caffeinilytica/caffeine/degradation/LysR family transcriptional reg-ulator factor分类
生物科学引用本文复制引用
朱浩,张文泽,高子晴..CdnR2对Paraburkholderia caffeinilytica CF1咖啡因降解基因簇的转录调控[J].大连工业大学学报,2025,44(5):332-339,8.基金项目
辽宁省教育厅高等学校基本科研项目(LJKMZ20220879). （LJKMZ20220879）
