神经损伤与功能重建2025，Vol.20Issue(10)：559-563,5.DOI:10.16780/j.cnki.sjssgncj.20241082
强啡肽A(1-8)纳米颗粒的脑靶向性和外周毒性研究
Study on Brain Targeting and Peripheral Toxicity of Deltorphin A(1-8)Nanoparticles
摘要
Abstract
Objective:This study aimed to verify the brain targeting and peripheral toxicity of Deltorphin A(1-8)[DYN-A(1-8)]nanoparticles in rats with cerebral ischemia-reperfusion injury.Methods:DYN-A(1-8)was encapsulated into poly(citric acid)-g-arginine(PCGA)based nanoparticles,and the characteristics of DYN-PCGA nanoparticles were evaluated.Fluorescent Cy5-labeled DYN-A(1-8)or DYN-PCGA was injected into middle cerebral artery occlusion(MCAO)rats via the tail vein,and an in vivo imaging system was used to validate the brain targeting ability of DYN-PCGA.The effects of DYN-A(1-8)nanoparticles on the histopathology of heart,liver,spleen,lung,and kidney tissues in MCAO rats were observed by HE staining to assess the in vivo toxicity of DYN-PCGA administered via the tail vein.Results:The characterization results showed that DYN-PCGA(1∶5)exhibited small particle size,high stability,and strong drug loading capacity.In vivo imaging results indicated that DYN-PCGA could effectively transport drugs across the blood-brain barrier to the brain after administration,demonstrating good brain targeting.HE staining results revealed that the tissue sections of heart,liver,spleen,lung,and kidney in MCAO rats showed normal cellular structure without edema or inflammatory cell infiltration,and no abnormal histopathological changes were found.Conclusion:DYN-PCGA nanoparticles extended the plasma half-life of DYN-A(1-8),exhibiting good brain targeting and certain safety.关键词
强啡肽A(1-8)/纳米颗粒/脑靶向性/毒性Key words
deltorphin A(1-8)/nanoparticles/brain targeting/toxicity分类
医药卫生引用本文复制引用
陈梦萦,展淑琴..强啡肽A(1-8)纳米颗粒的脑靶向性和外周毒性研究[J].神经损伤与功能重建,2025,20(10):559-563,5.基金项目
国家自然科学基金项目(前蛋白转化酶PC1及PCSK9在脑缺血时的变化及作用研究,No.81070999) （前蛋白转化酶PC1及PCSK9在脑缺血时的变化及作用研究,No.81070999）
2022年度陕西省卫生健康科研项目(沉默PCSK9通过GPX4/ACSL4信号抑制铁死亡逆转脑缺血再灌注损伤的作用及机制,No.2022A006) （沉默PCSK9通过GPX4/ACSL4信号抑制铁死亡逆转脑缺血再灌注损伤的作用及机制,No.2022A006）
