高原农业2025，Vol.9Issue(5)：605-616,662,13.DOI:10.19707/j.cnki.jpa.2025.05.008
云南沙棘三萜类化合物提取纯化工艺研究
A Study of Extraction and Purification Process of Triterpenoids from Seabuckthorn Fruits in Yunnan
摘要
Abstract
Yunnan sea buckthorn is rich in triterpene acids with hypoglycemic activity.In order to establish the extraction and purification process of hypoglycemic active ingredients in Tibetan seabuckthorn,this study used Yunnan seabuckthorn fruit as raw material to obtain triterpenoid acid by ethanol extraction and AB-8 macroporous resin purification.The results showed that the optimum extraction conditions were as follows:liquid-solid ratio 40 mL/g,ethanol concentration 65%,extraction temperature 57℃,extraction time 30 min.The optimum adsorption and desorption conditions of AB-8 macroporous resin were as follows:the sample concentration was 20 mg/mL,the pH was 6,the adsorption flow rate was 2.5 mL/min,and the ethanol concentration was 55%.After purification,the purity increased by 2.49 times.This method proved stable and feasible,and can be used for the extraction and purification of triterpene acids from Yunnan seabuckthorn.关键词
沙棘/三萜类化合物/提取纯化/均匀试验Key words
Seabuckthorn/Triterpenic acid/Extraction and purification/Homogeneous test分类
轻工纺织引用本文复制引用
刘青松,刘子微,金惠雅,鲁东泽,曾庆熙,钟政昌..云南沙棘三萜类化合物提取纯化工艺研究[J].高原农业,2025,9(5):605-616,662,13.基金项目
西藏科技(XZ202201ZD0001N) （XZ202201ZD0001N）
西藏农牧学院研究生创新计划资助项目(YJS2024-52). （YJS2024-52）
