高原农业2025，Vol.9Issue(5)：625-636,12.DOI:10.19707/j.cnki.jpa.2025.05.010
青稞根际拮抗菌筛选及其发酵液的抑菌活性研究
Screening of Rhizobacteria from the Rhizosphere of Highland Barley and the Antibacterial Activity of Their Fermentation Broths
摘要
Abstract
Barley(Hordeum vulgare L.var.nudum)is a major food crop in Xizang,widely cultivated on the Tibetan Plateau,with important economic and ecological values.However,the unique environment of Xizang has resulted in the frequent occurrence of wheat diseases,and the excessive use of chemical fertilizers and pesticides has seriously threatened the ecology.This study aimed to explore the environmentally friendly biological control method,from the barley inter-root soil screening antagonistic strains,study fermentation filtrate characteristics and the promotion of the characteristics of the screening of good strains,through the plate standoff method to assess the inhibitory activity,and provide support for the development of barley special microbial fertilizers.The results showed:Five strains with significant antagonistic effects against Fusarium graminearum and Fusarium solani were isolated from healthy barley rhizosphere soil.Morphological observation and 16S rRNA gene sequencing identified them as Pseudomonas sp.QK-1,Alcaligenes sp.QK-7 and QK-32,Stenotrophomonas sp.QK-17,and Bacillus sp.QK-18;Fermentation filtrate tests revealed that strains QK-7 and QK-17 exhibited significant inhibitory effects on target pathogens with 76.66%and 90.84%,with stable activity within the temperature range of 60 ℃～80℃;Comprehensive evaluation of antimicrobial activity and growth-promoting traits indicated that strain QK-7 demonstrated the most promising biocontrol potential.These findings provide valuable microbial resources and technical support for developing biocontrol agents suitable for cereal crops in Xizang.关键词
青稞/根际拮抗菌/发酵滤液/抑菌活性/促生特性/生物防治Key words
Barley/Rhizosphere antagonistic bacteria/Fermentation filtrate/Antimicrobial activity/Growth-promoting traits/Biocontrol分类
农业科技引用本文复制引用
次仁央金,夏洪艳,赵为民,魏丽萍,巩文峰..青稞根际拮抗菌筛选及其发酵液的抑菌活性研究[J].高原农业,2025,9(5):625-636,12.基金项目
"新农科"高原植物生产类专业提升实践创新能力平台建设(藏财预指 2023-1 号) （藏财预指 2023-1 号）
西藏自治区重点研发计划项目(XZ202201ZY0011N) （XZ202201ZY0011N）
野生食药用菌资源保护与利用跨区域合作科技创新中心,编号,YDZJ202402013CXJD. （）
