海南医科大学学报2025，Vol.31Issue(19)：1441-1448,8.DOI:10.13210/j.cnki.jhmu.20241127.001
LncRNA MIAT/miR-150-5p/NEGR1分子轴在大鼠脊髓缺血再灌注损伤中的表达
Expression of the LncRNA MIAT/miR-150-5p/NEGR1 axis in rat spinal cord ischemia-reperfusion injury
摘要
Abstract
Objective:To investigate the expression and possible mechanisms of lncRNA MIAT/miR-150-5p/NEGR1 axis in rat spinal cord ischemia-reperfusion injury(SCIRI).Methods:A SCIRI rat model was established,and 18 SD rats were divided into the Sham group and the SCIRI model(I/R)group,with 9 rats in each group.Neurological function score was used to evalu-ate hind limb motor activity in rats.H&E staining and Nissl staining were used to observe the pathological changes of spinal cord tissue and the morphology and structure of neuron Nissl bodies.qRT-PCR was used to detect the mRNA expression of lncRNA MIAT,miR-150-5p,and NEGR1 in spinal cord tissue.The targeted regulatory relationship between lncRNA MIAT and miR-150-5p as well as miR-150-5p and NEGR1 was verified by Starbase database,TargetScan7.2 target gene prediction website,and double luciferase reporter gene assay.Results:Compared to the Sham group,the Basso-Beattie-Bresnahan score was significantly lower(P<0.05),neuronal injury was observed in spinal cord tissue,the number of Nissl bodies decreased,the expression of ln-cRNA MIAT and NEGR1 were significantly up-regulated(P<0.05),and the expression of miR-150-5p was significantly down-regulated(P<0.05)in I/R group.Dual luciferase reporter gene detection results showed that there were targeted regulatory rela-tionships between lncRNA MIAT and miR-150-5p as well as miR-150-5p and NEGR1.Conclusion:LncRNA MIAT is highly ex-pressed in SCIRI model rats,which may be involved in the occurrence and development of SCIRI by regulating miR-150-5p/NE-GR1 axis.关键词
脊髓缺血再灌注损伤/lncRNA MIAT/miR-150-5p/NEGR1Key words
Spinal cord ischemia-reperfusion injury/lncRNA MIAT/miR-150-5p/NEGR1分类
医药卫生引用本文复制引用
王峥,马梦洁,刘建光,杨秋香..LncRNA MIAT/miR-150-5p/NEGR1分子轴在大鼠脊髓缺血再灌注损伤中的表达[J].海南医科大学学报,2025,31(19):1441-1448,8.基金项目
This study was supported by the Natural Science Foundation Project of Hubei Province(2019CFB762) 湖北省自然科学基金资助项目(2019CFB762) （2019CFB762）
