海南医科大学学报2025，Vol.31Issue(19)：1449-1457,9.DOI:10.13210/j.cnki.jhmu.20241029.001
RECK基因对肝癌细胞系MHCC97-H/L表型作用的影响
Effect of RECK gene on the phenotype of liver cancer cell line MHCC97-H/L
摘要
Abstract
Objective:To investigate the effect of RECK expression levels on the proliferation,apoptosis,invasion and migra-tion ability of human liver cancer cell line MHCC97-H/L.Methods:Firstly,the RNA-seq data of hepatocellular carcinoma sam-ples in TCGA and GTEx databases were analyzed by bioinformatics to screen out a key gene RECK related to tumor metastasis.Then,RECK interference and overexpression models were constructed in MHCC97-H and MHCC97-L cells by lentiviral trans-fection,respectively.The mRNA and protein expression of RECK in MHCC97H/L cells were detected by qRT-PCR and West-ern blot,respectively.EDU method was used to detect cell proliferation.Flow cytometry analysis was used to detect cell apopto-sis.Transwell migration assay was used to detect cell invasion.Cell scratch assay was used to detect cell migration.Gelatin en-zyme spectrum,ELISA,and Western blot were used to detect the expression of MMP-2,MMP-9 and MT1-MMP in MHCC97-H/L cells.Results:The expression of RECK showed statistically significant differences(P<0.05)in G1-G4 stages(es-pecially in G3 stage)of hepatocellular carcinoma samples.Compared to the Sh-NC group,increased proliferation ability,cell inva-sion and migration,and decreased cell apoptosis in MHCC97-L cells were observed in Sh-RECK group with a significant differ-ence(P<0.05).Compared to the Oe-NC group,decreased proliferation ability,cell invasion and migration,and increased cell apoptosis in MHCC97-H cells were observed in the Oe-RECK group with a significant significance(P<0.05).Further research showed that the Sh-RECK group significantly promoted the expression of MMP-2,MMP-9 and MT1-MMP in MHCC97-L cells.While the Oe-RECK group inhibited the expression of these MMPs in MHCC97-H cells,which showed a statistically significant difference(P<0.05).Conclusion:The expression of RECK can enhance apoptosis of liver cancer cells and reduce their prolifera-tion,invasion,and migration abilities.关键词
RECK基因/肝细胞癌/转移/表型Key words
RECK gene/Hepatocellular carcinoma/Metastasis/Phenotype分类
医药卫生引用本文复制引用
丁碧莹,王利新,董辉..RECK基因对肝癌细胞系MHCC97-H/L表型作用的影响[J].海南医科大学学报,2025,31(19):1449-1457,9.基金项目
This study was supported by the National Natural Science Foundation of China Youth Program(82002955) （82002955）
the Natural Science Foundation of Ningxia Hui Autonomous Region(2022AAC03567) 国家自然科学基金青年项目(82002955) （2022AAC03567）
宁夏自然科学基金资助项目(2022AAC03567) （2022AAC03567）
