海南医科大学学报2025，Vol.31Issue(19)：1469-1476,8.DOI:10.13210/j.cnki.jhmu.20250102.001
青蒿素通过改善心肌线粒体功能减轻脓毒症小鼠心肌损伤的实验研究
Experimental studies of artemisinin-attenuated myocardial injury in sepsis-induced mice by improving myocardial mitochondrial function
摘要
Abstract
Objective:To explore the effect of artemisinin(ART)in reducing myocardial injury in mice caused by different strains.Methods:Atotal of 104 C57/BL mice were randomized to the sham surgery group(sham group),the Gram positive/negative sepsis group(G+/G-sepsis group),and the Gram positive/negative sepsis+ART group(ART 5、10、15、20、25+G+/G-sepsis).Take samples 12 h after molding,myocardial pathological changes were observed and scored by H&E stain-ing,and mouse serum CK-MB and cTnⅠ concentrations were determined by ELISA.The mechanism of ART in alleviating myocardial injury in septic mice was explored in the ART 25 mg/kg dose group.The LVEF,LVFS,LVEDD,LVESD,EDV and ESV levels of the mice hearts were detected by cardiac ultrasound,and the myocardial mitochondrial damage was observed and scored by transmission electron microscopy.Results:Compared to the sham group,the G+/G-sepsis group had severe myocardial injury and inflammatory cell infiltration,CK-MB and cTnⅠ,which were significant(P<0.05).Each dose group of ART significantly reduced cardiac muscle fiber injury and inflammatory cell infiltration,and the injury score,serum CK-MB and cTnⅠ levels showed a concentration-dependent significantly decrease compared to G+/G-sepsis group(P<0.05);compared to the sham group,the G+/G-sepsis group showed decreased myocardial systolic function,severe myocardial mitochondria,and significant morphological damage and score(P<0.05);Compared to the G+/G-sepsis group,the ART 25+G+/G-sepsis group had improved myocardial systolic function and mitochondrial damage score decreased scores,and only a small part of the mitochondrial morphology was disrupted,showing a statistically significant difference(P<0.05).Conclusion:Artemisinin has a protective effect on myocardial injury in Staphylococcus aureus/Escherichia coli-induced sepsis in mice,and the mechanism may be related to improve myocardial mitochondrial function.关键词
脓毒症/心肌损伤/青蒿素/心肌线粒体Key words
Sepsis/Myocardial injury/Artemisinin/Myocardial mitochondria分类
医药卫生引用本文复制引用
张琪,赵长甲,姜波,薛庆,许航,任珊..青蒿素通过改善心肌线粒体功能减轻脓毒症小鼠心肌损伤的实验研究[J].海南医科大学学报,2025,31(19):1469-1476,8.基金项目
This study was supported by the Eighth Division Shihezi City Young and Middle-Aged Science and Technology Innovation Leading Talent Plan Project(2020RC001) （2020RC001）
Central Government Guidance Fund for Local Science and Technology Development-Xinjiang Production and Construction Corps Clinical Medical Research Center(Bingkefa 2022-6) （Bingkefa 2022-6）
Xinjiang Production and Construction Corps Guidance Project(2023ZD020,2023ZD003) 八师石河子市中青年科技创新领军人才计划项目(2020RC001) （2023ZD020,2023ZD003）
中央引导地方科技-新疆生产建设兵团危重症疾病临床医学研究中心(兵科发2022-6) （兵科发2022-6）
兵团指导性项目(2023ZD020,2023ZD003) （2023ZD020,2023ZD003）
