脊蛇祛湿胶囊对类风湿关节炎合并骨质疏松症患者骨保护作用机制的网络药理学及回顾性研究
The bone-protective mechanisms of Jishe Qushi Capsules in patients with rheumatoid arthritis complicated by osteoporosis:A network pharmacology and retrospective study
摘要
Abstract
Objective:To investigate the bone-protective mechanisms of Jishe Qushi Capsule in patients with rheumatoid ar-thritis(RA)combined with osteoporosis using network pharmacology and molecular docking techniques,and to verify these find-ings through a retrospective analysis.Methods:The active ingredients and corresponding targets of the drug were obtained through the TCMSP,BATMAN-TCM,SwissTargetPrediction databases,and relevant literature.Disease-related targets were identified from the GeneCard,OMIM,and TTD databases.Intersection targets were determined using Venny 2.1.0,and the protein-protein interaction(PPI)network was constructed using Cytoscape 3.7.2 and the STRING database.Gene Ontology(GO)functional and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed using the DAVID database.Molecular docking of the main active components and targets was conducted using AutoDock Vina 1.1.2.A retrospective analysis was carried out on patients with RA combined with osteoporosis who were treated at the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from January 2018 to April 2024.The control group(n=81)and the experimental group(n=81)were compared in terms of general information and bone metabolism markers before and after treatment.Results:Jishe Qushi Capsule contained 142 active ingredients,which acted on key targets such as TP53,STAT3,AKT1,SRC,and JUN,exerting bone-protective effects by regulating the PI3K-Akt and MAPK signaling pathways.Molecular docking results showed that the main active components had strong binding affinities with core targets.The retrospective analysis demonstrated that the Δ25-(OH)D3 levels were significantly higher in the experimental group compared to the control group(P<0.05).Furthermore,the combination of"Jishe Qushi Capsule,Alfacalcidol Soft Capsule,Calcium Carbonate and Vitamin D3 Tab-let,and Vitamin D2 Injection"was found to be the most effective regimen(P<0.05).Conclusion:Jishe Qushi Capsule may have a potential bone-protective effect in RA patients with osteoporosis and may produce synergistic effects when combined with anti-osteoporotic medications.关键词
网络药理学/分子对接/脊蛇祛湿胶囊/类风湿关节炎/骨质疏松症Key words
Network pharmacology/Molecular docking/Jishe Qushi Capsules/Rheumatoid arthritis/Osteoporosis分类
医药卫生引用本文复制引用
季诗煜,黄春霞,袁雪梅,罗丰,王秋燚,马武开,姚血明..脊蛇祛湿胶囊对类风湿关节炎合并骨质疏松症患者骨保护作用机制的网络药理学及回顾性研究[J].海南医科大学学报,2025,31(19):1502-1512,11.基金项目
This study was supported by National Natural Science Foundation of China(82160869,82374494) （82160869,82374494）
Guizhou Province Basic Research(Natural Science)[Qiankehe Basic Research-ZK(2023)General 412,Guikehe Academic New Seedling(2023-36)] （Natural Science）
the Second Affiliated Hospital of Guizhou University of Chinese Medicine-Young Seedling Project[GZEYK-Y(2022)32] （2022）
Youth Science and Technology Talent Growth Project of Guizhou Provincial Department of Education[Qian Jiao Ji(2024)No.122] （2024）
National and Provincial Science and Technology Innovation Talent Team Cultivation Project of Guizhou University of Chinese Medicine[Guizhou University of Chinese Medicine TD Joint Word(2022)No.004] （2022）
Research Project of Science and Technology of Traditional Chinese Medicine and Ethnic Medicine of Guizhou Provincial Administration of Traditional Chinese Medicine(QZYY-2024-009) （QZYY-2024-009）
Key Laboratory of Translational Medicine for Integrated Traditional Chinese and Western Medicine in Disease Prevention and Treatment of Higher Education Institutions in Guizhou Province[Qian Jiao Ji(2023)No.017] 国家自然科学基金资助项目(82160869,82374494) （2023）
贵州省基础研究(自然科学)[黔科合基础-ZK(2023)一般412,贵科合学术新苗(2023-36号)] （自然科学）
贵州中医药大学第二附属医院-青苗项目[GZEYK-Y(2022)32] （2022）
贵州省教育厅青年科技人才成长项目[黔教技(2024)122号) （2024）
贵州中医药大学国家与省级科技创新人才团队培育项目[贵中医TD合字(2022)004号] （2022）
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2024-009) （QZYY-2024-009）
贵州省高等学校中西医结合防治疾病转化医学重点实验室[黔教技(2023)017号] （2023）
