河南农业大学学报2025，Vol.59Issue(5)：796-804,9.DOI:10.16445/j.cnki.1000-2340.20250126.001
玉米自交系ldhs1叶片早衰特性分析及QTL定位
Characteristics analysis and QTL mapping of early leaf senescence traits of maize inbred line ldhs1
摘要
Abstract
[Objective]This research was conducted to reveal the characteristics and genetic basis of maize leaf senescence and lay the groundwork for enhancing the genetic pool and guiding the breeding programs to improve or create new plant strains.[Method]An inbred line named ldhs1 was obtained from the DH lines created by maize haploid breeding technology.Observations of physical traits,mea-surements of physiological and biochemical properties,and examinations of tissue and cellular struc-tures were conducted,and the genes related to early leaf senescence in maize were located by using for-ward genetics and bulked segregant analysis sequencing(BSA-seq).[Result]Compared with the normal stay-green inbred line L521,the ldhs1 exhibited top-down early senescence from the 10th day after pollination,and gradually spread from leaf edge yellowing to the interior to the whole leaf yellow-ing.The leaves showed a reduction in vital chlorophyll levels and the activities of SOD and POD,coupled with heightened MDA content,which in turn negatively impacted the efficiency of photosyn-thesis and led to chloroplast dysplasia.Based on genetic analysis and BSA-seq,the maize early leaf senescence gene LDHS1 was located in a region of 1.06 Mb between molecular marker indel-12 and indel-13 of chromosome 1.[Conclusion]A novel inbred line with early leaf senescence from top to bottom was discovered,and the quantitative trait locus,(QTL)mapping of this trait was carried out and the related candidate genes were branded.关键词
玉米/自交系/早衰/生理特性/数量性状位点定位Key words
maize/inbred line/early senescence/physiological characteristics/QTL mapping分类
农业科技引用本文复制引用
马苗苗,张琪凡,郑斌瑞,张亚州,张利华,李星星,刘宗华,宋允允,李浩川..玉米自交系ldhs1叶片早衰特性分析及QTL定位[J].河南农业大学学报,2025,59(5):796-804,9.基金项目
河南省农业良种联合攻关项目(2022010202) （2022010202）
河南省科技攻关项目(222102110276) （222102110276）
国家重点研发计划项目(2016YFD0101205-3) （2016YFD0101205-3）
