河南农业科学2025，Vol.54Issue(10)：71-80,10.DOI:10.15933/j.cnki.1004-3268.2025.10.008
2株丹参根内促生细菌的分离鉴定及促生作用评价
Isolation,Identification and Effect Evaluation of Two Growth-promoting Endophytic Bacteria in Salvia miltiorrhiza Roots
摘要
Abstract
In order to obtain endophytic bacteria to promote growth and increase the yield of Salvia miltiorrhizae,two endophytic bacteria strains Kv13-2 and Ko 15-1 with growth-promoting effects were isolated and screened from the healthy roots of S.miltiorrhiza by tissue grinding and culture.Based on morphological and molecular biological analysis,isolates Kv13-2 and Ko 15-1 were identified as Klebsiella variicola and Kosakonia oryzae,respectively.The results of biomass promotion test showed that isolates Kv13-2 and Ko 15-1 could produce indole-3-acetic acid(IAA),and had the characteristics of phosphorus solubilization,potassium solubilization,and nitrogen fixation.The results of the seed germination experiment showed that at appropriate concentrations,inoculation with bacterial suspensions of Kv13-2 and Ko15-1 increased the germination rate of S.miltiorrhiza seeds by 92.38％and 129.72％,the plant height by 148.72％and 48.72％,and the root length by 102.86％and 90.02％,respectively.Pot experiment results showed that root irrigation with isolates Kv13-2 and Ko15-1 increased the fresh weight of S.miltiorrhiza stems and leaves by 200.00％and 104.48％,respectively,and the fresh weight of roots by 57.89％and 34.74％,respectively.The results of field experiments demonstrated that root irrigation with the two growth-promoting bacterial suspensions increased root length by 18.03％and 10.97％,root diameter by 7.69％and 0.75％,lateral root number by 11.17％and 24.63％,and yield by 16.43％and 24.80％,respectively.In addition,the two growth-prompting isolates could also significantly reduce the incidence and disease index of S.miltiorrhiza root rot,and the control effectiveness of two isolates reached 65.57％and 68.61％,respectively.In conclusion,isolates Kv13-2 and Ko15-1 have good growth-promoting effects,and have good application potential in practical production.关键词
丹参/内生细菌/促生作用/吲哚乙酸/防腐效果Key words
Salvia miltiorrhiza/Endophytic bacteria/Growth-promoting effect/Indole-3-acetic acid/Disease prvention effect分类
农业科技引用本文复制引用
王飞,杨健,万修福,李绍建,杨瑾,李雪梦,文艺,高素霞,秦艳红,鲁书豪,赵莹,宋露洋..2株丹参根内促生细菌的分离鉴定及促生作用评价[J].河南农业科学,2025,54(10):71-80,10.基金项目
国家中药材产业技术体系项目(CARS-21) （CARS-21）
河南省中药材产业技术体系项目(HARS-22-11-Z1) （HARS-22-11-Z1）
河南省农业科学院新兴学科项目(2024XK06) （2024XK06）
