中医药导报2025，Vol.31Issue(9)：9-16,31,9.DOI:10.13862/j.cn43-1446/r.2025.09.002
井穴刺络放血调控Nrf2/ARE通路改善脑卒中大鼠认知功能的机制研究
Mechanistic Study on the Improvement of Cognitive Function in Stroke Rats by Jing-Well Points Acupuncture through Modulation of the Nrf2/ARE Pathway
摘要
Abstract
Objective:To investigate the effect of Jing-well points acupuncture on improving cognitive dysfunction in rats with stroke by activating the Nrf2/ARE signaling pathway and its impact on the inflammatory response.Methods:Totally 70 SD rats were randomly divided into blank group,sham-operation group,model group,Jing-Well points acupuncture group,ML385(Nrf2 inhibitor)group,Jing-Well points acupuncture+ML385 group,and Resveratrol(Nrf2 agonist)group.The blank group received no treatment,the sham-operation group underwent a sham operation,and the other groups were subjected to permanent ischemic stroke via middle cerebral artery occlusion(MCAO).The Jing-Well points acupuncture group was treated with Jing-Well points acupuncture for 3 days(once daily,alternating sides).The ML385 group received intraperitoneal injections of ML385[30 mg/(kg·d)]for 7 days.The Jing-Well points acupuncture+ML385 group received Jing-Well points acupuncture and intraperitoneal injections of ML385[30 mg/(kg·d)].The Resveratrol group received intraperitoneal injections of Resveratrol[40mg/(kg·d)]for 7 days.Neurological function was scored using the modified neurological severity score(mNss)on the first day after modeling and the day before tissue collection.Cognitive function was assessed using Y-maze and novel object recognition tests.Brain infarct area was evaluated by 2,3,5-triph-enyltetrazolium chloride(TTC)staining.Serum levels of interleukin-6(IL-6),interleukin-1 β(IL-1β),tumor necrosis factor-α(TNF-α),model driven architecture(MDA),glutathione(GSH)and superoxide dismutase(SOD)activity were measured by enzyme-linked immunosorbent assay(ELISA).Real-time PCR was used to detect levels of Nrf2 mRNA,HO-1 mRNA and NQO1 mRNA in the hippocampus,and Western blotting was used to assess protein levels of Nrf2,HO-1,and NQO1.Results:The Jing-Well points acupuncture group and Resveratrol group showed lower mNss than model group(P＜0.05),while higher spontaneous alternation percentages than model group(P＜0.01 or P＜0.05).The Resveratrol group showed higher cognitive index than model group(P＜0.05).The Jing-Well points acupuncture group,Jing-Well points acupuncture+ML385 group,and Resveratrol group exhibited smaller brain infarct areas than model group(P＜0.05).The ML385 group showed higher serum levels of IL-6,IL-1β and TNF-α than model group(P＜0.01);The Jing-Well points acupuncture group,Jing-Well points acupuncture+ML385 group,and Resveratrol group showed lower levels of IL-6,IL-1β and TNF-α than model group(P＜0.01).The Jing-Well points acupuncture group and Resveratrol group showed higher serum MDA,GSH levels and SOD activity than model group(P＜0.01),while the ML385 group and Jing-Well points acupuncture+ML385 group showed lower serum MDA,GSH levels and SOD activity than model group(P＜0.01).HE staining revealed reduced neuronal damage in the Jing-Well points acupuncture group,Jing-Well points acupuncture+ML385 group,and Resveratrol group,while the ML385 group showed aggravated damage.The Jing-Well points acupuncture group and Resveratrol group exhibited higher Nrf2 mRNA,HO-1 mRNA,and NQO1 mRNA and protein levels of Nrf2,HO-1 and NQO1 than model group(P＜0.01).Conclusion:Jing-Well points acupuncture can improve cognitive dysfunction in stroke rats by activating the Nrf2/ARE pathway.It upregulates HO-1/NQO1 to enhance antioxidant capacity,suppresses inflammatory factors,alleviates oxidative stress and neuroinflammation,and reduces infarct size.关键词
脑卒中/井穴刺络/认知功能障碍/核因子E2相关因子2/抗氧化反应原件信号通路/大鼠Key words
stroke/Jing-Well points acupuncture/cognitive impairment/nuclear factor erythroid 2-related factor 2/antioxidant response element signaling pathway/rat分类
医药卫生引用本文复制引用
蓝相洲,李铃佳,李叶舟,薛珂,陈苗,文志,陈青..井穴刺络放血调控Nrf2/ARE通路改善脑卒中大鼠认知功能的机制研究[J].中医药导报,2025,31(9):9-16,31,9.基金项目
2023年长沙市软科学项目(Kh2302034) （Kh2302034）
2024年湖南省自然科学基金-医卫行业联合基金(2024JJ9424) （2024JJ9424）
2023年湖南省中医药管理局项目(D2023018) （D2023018）
