中医药导报2025，Vol.31Issue(9)：32-37,54,7.DOI:10.13862/j.cn43-1446/r.2025.09.005
黄芪当归汤联合中医序贯疗法促进SD大鼠肛周脓肿术后创面愈合
Huangqi Danggui Decoction(黄芪当归汤)Combined with Traditional Chinese Medicine Sequential Therapy on Promoting Wound Healing after Perianal Abscess Surgery in SD Rats
摘要
Abstract
Objective:To determine whether Huangqi Danggui decoction combined with sequential dressing change can promote wound healing after perianal abscess surgery.Methods:Totally 24 SD rats were used to establish a postoperative model of perianal abscess and the rats were divided into model group,Huangqi Danggui decoction group,Traditional Chinese Medicine(TCM)sequential dressing change group(sequential group),and Huangqi Danggui decoction combined with TCM sequential dressing change group(combination group).Another 6 healthy SD rats were taken without any treatment as the normal group.Except for the normal group,the treatments were given once a day for a total of 21 days.The wound healing rates were measured at 7th day,14th day,and 21st day.HE staining was used to observe the wound tissue lesions.Enzyme-linked immunosorbent assay(ELISA)was used to detect the concentrations of tumor necrosis factor-α(TNF-α),inter-leukin-6(IL-6)and andinterleukin-10(IL-10)in wound tissue.The expression levels of vascular endothelial growth factor(VEGF)and vascular endothelial cell specific marker(CD31)in wound tissue were detected by immunohistochemistry.The number of CD86+macrophages and CD206+macrophages in wound tissue was detected by flow cytometry.Western blotting was used to detect the protein expressions of Janus kinase 2(JAK2),phospho-rylase Janus kinase 2(p-JAK2),signal transducer and activator of transcription 3(STAT3),and phosphorylase signal transducer and activator of transcription 3(p-STAT3)in wound tissue.Results:The wound tissue of the model group was not completely healed within 21 days,and most of the wound tissue of the other medication groups were completely healed.Compared with the normal group,the wound tissue structure of the model group was severely damaged,and the TNF-α,IL-6,VEGF and CD31 protein expressions were increased(P＜0.05).The number of CD86+macrophages were increased(P＜0.05),and the IL-10,JAK2,p-JAK2,STAT3 and p-STAT3 protein expressions were decreased in model group(P＜0.01).The number of CD206+macrophages was reduced in model group(P＜0.05).Compared with the model group,the Huangqi Danggui decoction group,sequential group and combination group showed significant improvement(P＜0.05),and the combination group showed the best effect(P＜0.01).Conclusions:The method of oral administration of Huangqi Danggui decoction and external application of TCM sequential dressing change,can promote wound angiogenesis and wound healing.关键词
肛周脓肿/创面愈合/黄芪当归汤/中医序贯疗法/血管新生/大鼠Key words
perianal abscess/wound healing/Huangqi Danggui decoction/traditional Chinese medicine sequential therapy/angiogenesis/rats分类
医药卫生引用本文复制引用
张永丽,钱海华,孙林梅,张欣颖,宫丹丹,胡建生..黄芪当归汤联合中医序贯疗法促进SD大鼠肛周脓肿术后创面愈合[J].中医药导报,2025,31(9):32-37,54,7.基金项目
陕西省重点研发计划项目(2023-YBSF-625) （2023-YBSF-625）
西安市科技计划项目(22YXYJ0058) （22YXYJ0058）
西安市科技局重点项目(24YXYJ0024) （24YXYJ0024）
