中医药导报2025，Vol.31Issue(9)：38-43,49,7.DOI:10.13862/j.cn43-1446/r.2025.09.006
小儿久咳合剂对感染后咳嗽大鼠SUMF1/SUMF2平衡的调节作用及对IL-13分泌的影响
Effect of Xiao'er Jiuke Mixture(小儿久咳合剂)on Balance of Sumf1/Sumf2 and Secretion of IL-13 in Infected Cough Rats
摘要
Abstract
Objective:To investigate the effect of Xiao'er Jiuke mixture on regulating the SUMF1/SUMF2 balance and its modulatory role on IL-13 secretion levels in rats with post-infectious cough(PIC).Methods:Totally 40 Sprague-Dawley(SD)rats were randomly assigned into blank control group(n=10),model group(n=10),Chinese medicine group(n=10),and Western medicine group(n=10).A PIC model was established using a combination of smoke exposure,lipopolysaccharide(LPS)nasal instillation,and capsaicin aerosol challenge.The Chinese medicine group was administered Xiao'er Jiuke mixture solution(1.80 g/mL)via oral gavage.The Western medicine group received Montelukast sodium solution(0.08 mg/mL)via oral gavage,and the blank control group and model group received an equal volume of 0.9％sodium chloride solution via oral gavage.Pathological changes in lung tissue were assessed using Masson,periodic acid-Schiff(PAS),and hematoxylin-eosin(HE)staining.Serum and bronchoalveolar lavage fluid(BALF)levels of total IgE,specific IgE,and IL-13 were measured by enzyme-linked immunosorbent assay(ELISA).The expression levels of SUMF1,SUMF2,and IL-13 in lung tissue were determined by Western blotting(WB)and polymerase chain reaction(PCR).Results:Compared to the blank control group,lung tissue sections from the model group exhibited significant tracheal epithelial squamous metaplasia,extensive inflammatory cell infiltration,and pulmonary interstitial expansion.Lung tissue levels of SUMF1,the SUMF1/SUMF2 ratio,and IL-13 were significantly increased in model group(P＜0.01),while SUMF2 levels were significantly decreased(P＜0.01).Neutrophil and macrophage densities were also significantly elevated in model group(P＜0.01).Furthermore,serum and BALF levels of total IgE,specific IgE,and IL-13 were all significantly increased in model group(P＜0.01)compared to the blank control.Compared to the model group,Chinese medicine group and Western medicine group showed significantly reduced inflammatory cell infiltration and tracheal epithelial damage in lung tissue.Levels of SUMF1,SUMF1/SUMF2 ratio,and IL-13 were significantly decreased in Chinese medicine group and Western medicine group(P＜0.01),while SUMF2 levels were significantly increased(P＜0.01).Inflammatory cell densities were also significantly reduced in Chinese medicine group and Western medicine group(P＜0.01).Serum and BALF levels of total IgE,specific IgE,and IL-13 were likewise significantly decreased in Chinese medicine group and Western medicine group(P＜0.01)compared to the model group.Conclusion:Xiao'er Jiuke Mixture may exert its therapeutic effect by restoring the balance of SUMF1/SUMF2,inhibiting the activity and secretion of IL-13,and reducing airway inflammation.关键词
感染后咳嗽/气道炎症/小儿久咳合剂/磷酸酯酶修饰因子/白细胞介素-13/大鼠Key words
coughing after infection/airway inflammation/Xiao'er Jiuke mixture/phosphatase modifying factor/interleukin-13/rat分类
医药卫生引用本文复制引用
李霁航,郭育辰,朱宇子,吴哲伟,周智,刘靖雷,罗银河..小儿久咳合剂对感染后咳嗽大鼠SUMF1/SUMF2平衡的调节作用及对IL-13分泌的影响[J].中医药导报,2025,31(9):38-43,49,7.基金项目
湖南省2022年大学生创新创业训练计划项目(2022-2886)(S202210541017) （2022-2886）
