中医药导报2025，Vol.31Issue(9)：44-49,6.DOI:10.13862/j.cn43-1446/r.2025.09.007
健脾醒酒方通过雌激素受体1调控PI3K/Akt通路改善酒精诱导的肝细胞损伤
Jianpi Xingjiu Recipe(健脾醒酒方)Improves Alcohol-Induced Liver Cell Damage by Regulating the PI3K/Akt Pathway through Estrogen Receptor1
摘要
Abstract
Objective:To study the regulation of Jianpi Xingjiu recipe through estrogen receptor 1(ESR1)by phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway to explore its effect on alcohol-induced liver cell injury in mice and its mechanism of action.Methods:Totally 60 SPF grade BALB/c male mice were adaptively raised for 1 week and randomly divided into blank group,model group,Jianpi Xingjiu recipe low-dose group,Jianpi Xingjiu recipe high-dose group,positive drug group 1 and positive drug group 2,10 rats in each group.Except for the blank group,each group was fed deionized water containing ethanol.From the 2nd to the 5th day,the ethanol concentration gradually increased from 1％to 4％,and from the 6th to the 13th day,the ethanol concentration was 5％.Starting from the 7th day,0.5 mL of 20％ethanol was administered by intragastric administration,and the non-alcohol feeding group received an equal volume of deionized water.Starting from the 8th day,the mice in each group were administered with corresponding drugs for 7 days,and the blank group and model group were administered with an equal volume of deionized water.The eyeballs were removed to collect blood 0.5 h after the last gastric administration.The mice were sacrificed and the liver and stomach were dissected and removed.Gas chromatography was used to determine ethanol content,and Reich's method was used to detect alanine aminotransferase(ALT)and aspartate aminotransferase(AST).Colorimetric method was used to detect alcohol dehydrogenase(ADH)and aldehyde dehydrogenase(ALDH)levels.Enzyme-linked immunosorbent assay(ELISA)method was used to detect superoxide dismutase(SOD),malondialdehyde(MDA),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α).Western blotting method was used to detect the expression levels of PI3K,Akt,p-PI3K,p-Akt,ESR1 and MMP-2 in liver tissue and the expression level of PTPN11 in gastric tissue.The pathological sections of mouse liver and stomach tissue were observed with an optical microscope.Results:The Jianpi Xingjiu recipe low-dose group,Jianpi Xingjiu recipe high-dose group and positive drug group 1 showed lower blood ethanol contents than model group(P＜0.05).Compared with the model group,ADH and ALDH increased in Jianpi Xingjiu recipe low-dose group,Jianpi Xingjiu recipe high-dose group and positive drug group 1,while ALT and AST decreased(P＜0.05).Compared with the model group,the IL-6,TNF-α and MDA decreased in Jianpi Xingjiu recipe low-dose group,Jianpi Xingjiu recipe high-dose group,positive drug group 1 and positive drug group 2,while SOD increased(P＜0.05).Compared with the model group,the protein expressions of ESR1,MMP-2,PTPN11,p-PI3K/PI3K,and p-Akt/Akt were reduced in Jianpi Xingjiu recipe low-dose group and Jianpi Xingjiu recipe high-dose group(P＜0.05),and liver and stomach tissue pathological morphology showed that the pathological conditions were significantly improved in Jianpi Xingjiu recipe high-dose group.Conclusion:Jianpi Xingjiu recipe may inhibit MMP-2 and PTPN11 protein expression through the PI3K/Akt signaling pathway regulated by ESR1,thereby reducing blood ethanol content,alleviating alcohol-induced liver and gastric mucosal inflammatory damage,and alleviating mild alcoholism.关键词
酒精性肝损伤/健脾醒酒方/雌激素受体1/磷脂酰肌醇3-激酶/蛋白激酶B信号通路/小鼠Key words
alcoholic liver injury/Jianpi Xingjiu recipe/estrogen receptor1/phosphatidylinositol 3-kinase/protein kinase B pathway/mice分类
医药卫生引用本文复制引用
李堃,周广泉,杨琰,葛飞..健脾醒酒方通过雌激素受体1调控PI3K/Akt通路改善酒精诱导的肝细胞损伤[J].中医药导报,2025,31(9):44-49,6.基金项目
南通市中医医疗联盟课题(TZYK202318) （TZYK202318）
南通市科学技术局课题(MSZ21006) （MSZ21006）
