中医药导报2025，Vol.31Issue(9)：50-54,5.DOI:10.13862/j.cn43-1446/r.2025.09.008
"返魂草-法半夏"配伍治疗寒饮蕴肺型慢性支气管炎大鼠的药效作用研究
Study on Fanhuncao(Aleutian Ragwort)-Fabanxia(Pinelliae Rhizoma Praeparatum)in Treatment of Rats with Chronic Bronchitis with Syndrome of Cold Fluid Retention
摘要
Abstract
Objective:To explore the pharmacological effects of compatibility of Fanhuncao(aleutian ragwort)-Fabanxia(pinelliae rhizoma praeparatum)(AP)in the treatment of chronic bronchitis(CB)with syndrome of cold fluid retention in rats.Methods:The CB model with syndrome of cold fluid retention in SD rats was established by smoke inhalation.The rats were randomly divided into normal control group,model group,positive control group(Lung Ning granules,2.70 g/kg),and high,medium,and low dose groups of AP(5.40,2.70 and 1.35 g/kg).The changes in body weight,body temperature,food intake,water intake,and organ coefficient(lung)of the rats were observed and recorded.The expectorant was detected by the phenol red method.The expression levels of tumor necrosis factor-α(TNF-α),transforming growth factor-β(TGF-β),interleukin-2(IL-2),interleukin-4(IL-4),malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by enzyme-linked immunosorbent assay(ELISA).The morphological changes of lung tissue were detected by hematoxylin-eos(HE)staining.Results:Compared with the normal control group,the body weight,food intake,water intake,phenol red excretion,and the levels of IL-2 and SOD significantly decreased in model group(P＜0.05),while the organ coefficient(lung)and the levels of TNF-α,TGF-β,IL-4 and MDA significantly increased(P＜0.05).Compared with the model group,the high-dose group of AP significantly increased the body weight,food intake,water intake,phenol red excretion,and the levels of IL-2 and SOD of the rats(P＜0.05),while decreased the organ coefficient(lung),the levels of TNF-α,TGF-β,IL-4,and MDA(P＜0.05).Compared with the positive control group,there was no significant difference in the body weight,food intake,water intake,phenol red excretion,organ coefficient(lung),IL-4,IL-2,SOD,and MDA levels of the rats the high-dose group of AP(P＞0.05),while the high-dose group of AP showed lower levels of TNF-α and TGF-β than positive control group(P＜0.05).Conclusion:The high-dose AP may play a role in the treatment of CB with syndrome of cold fluid retention in rats by decreasing levels of TNF-α,TGF-β,IL-4,MDA,increasing IL-2 and SOD,reducing pulmonary inflammation and enhancing antioxidant capacity.Moreover,the effect of high-dose AP in controlling the levels of TNF-α and TGF-β in CB with syndrome of cold fluid retention rats was superior to that of Lung Ning granules.关键词
慢性支气管炎/返魂草/法半夏/配伍/寒饮蕴肺型/炎症/大鼠Key words
chronic bronchitis/Fanhuncao(aleutian ragwort)/Fabanxia(pinelliae rhizoma praeparatum)/compatibility/cold fluid retention in lung/inflammation/rat分类
医药卫生引用本文复制引用
王利,李辰辉,龚志悦,黄玉婷,李娟.."返魂草-法半夏"配伍治疗寒饮蕴肺型慢性支气管炎大鼠的药效作用研究[J].中医药导报,2025,31(9):50-54,5.基金项目
湖南中医药大学重点学科中药学科资助项目(校行发规字[2023]2号) （校行发规字[2023]2号）
