中医药导报2025，Vol.31Issue(9)：55-60,6.DOI:10.13862/j.cn43-1446/r.2025.09.009
基于TNF信号通路探讨三痹颗粒治疗类风湿关节炎模型大鼠的机制
The Mechanism of Sanbi Granules(三痹颗粒)in Treating Rheumatoid Arthritis Model Rats Based on TNF Signaling Pathway
摘要
Abstract
Objective:To explore the effect and possible mechanisms of Sanbi granules on rheumatoid arthritis(RA)model rats.Method:RA model was established by injecting complete Freund's adjuvant and BCG oil emulsion into the subcutaneous layer of rat tail root using injection method.The rats were randomly divided into model control group,methotrexate group,high dose group of Sanbi granules,medium dose group of Sanbi granules,and low dose group of Sanbi granules,with 10 rats in each group.A blank control group was also established.The high,medium,and low dose groups of Sanbi granules were given 5.40 g/kg,2.70 g/kg,and 1.35 g/kg of Sanbi granules,respectively,3 times a day.The methotrexate group was given methotrexate(0.90mg/kg),twice a week.The blank control group and model control group were given distilled water at a dose of 10 mL/kg,3 times a day.After continuous administration for 4 weeks,the swelling degree of the ankle joint in rats was detected and HE staining was implemented.The enzyme-linked immunosorbent assay(ELISA)kit was used to measure serum tumor necrosis factor(TNF)-α,interleukin-6(IL-6),interleukin-17(IL-17),and C-reactive protein(CRP).Western blotting analysis was used to measure the protein relative expression levels of TNF-α,IL-6,IL-17,and CRP in toe tissue.Real-time quantitative polymerase chain reaction(PCR)was used to measure the relative expression levels of TNF-α mRNA,IL-6 mRNA,IL-17 mRNA,and CRP mRNA.Results:Compared with the model control group,the ankle joint swelling degree was reduced in high,medium,and low dose groups of Sanbi granules and methotrexate group(P＜0.01),and the inflammatory cell infiltration was reduced.At the same time,the TNF-α protein and TNF-α mRNA relative expression levels reduced in the high-dose group of Sanbi granules and methotrexate group(P＜0.05),indicating that the TNF signaling pathway was inhibited.The protein and mRNA relative expression levels of downstream inflammatory factors IL-6,IL-17,and CRP were also significantly reduced(P＜0.05).In addition,compared with the methotrexate group,there was no statistically significant difference in ankle joint swelling and protein and mRNA relative expression levels of TNF-α,IL-17 and IL-6 in high-dose group of Sanbi granules(P＞0.05).Conclusion:Sanbi granules can inhibit the TNF signaling pathway by reducing the expression level of TNF-α,and further down-regulate the expression level of downstream inflammatory factors,alleviate the inflammatory cell infiltration and ankle joint swelling that occur during the development of RA,and reduce the inflammatory response.关键词
类风湿关节炎/三痹颗粒/TNF信号通路/炎症反应/炎症因子Key words
rheumatoid arthritis/Sanbi granules/TNF signaling pathway/inflammatory response/inflammatory factors分类
医药卫生引用本文复制引用
尹玲桃,谢湘雄,禹勇军,陈达,杨蕙..基于TNF信号通路探讨三痹颗粒治疗类风湿关节炎模型大鼠的机制[J].中医药导报,2025,31(9):55-60,6.基金项目
湖南省中医药管理局项目(B2024158) （B2024158）
娄底市科学技术局2024年度临床医疗技术创新专项(娄科发[2024]17号) （娄科发[2024]17号）
2025年度娄底市哲学社会科学成果评审委员会课题(202535B) （202535B）
