江汉大学学报（自然科学版）2025，Vol.53Issue(5)：16-22,7.DOI:10.16389/j.cnki.cn42-1737/n.2025.05.002
油菜EMS诱变群体萌发期耐盐突变体筛选与鉴定
Screening and Identification of Salt-tolerant Mutants in the Germination Stage of Rapeseed EMS Mutagenesis Population
摘要
Abstract
276 stable M4 lines derived from the rapeseed cultivar'Zhongshuang 11'via ethyl methanesulfonate(EMS)mutagenesis were subjected to precise salt-tolerance screening at the germination stage,which identified 29 mutant lines with significantly enhanced salt-tolerance.Among these,6 mutants exhibited salt-tolerance comparable to the salt-tolerant cultivar'Huayouza 62'at the germination stage,while 2 mutants outperformed'Huayouza 62'in salt-tolerance.Additionally,this study optimized a technical workflow for improving rapeseed salt-tolerance through EMS mutagenesis.This work marks the first exploration of EMS mutagenesis for enhancing salt-tolerance in rapeseed,providing valuable germplasm resources and a technical pathway for rapid breeding of saline-alkali-tolerant rapeseed varieties.关键词
甘蓝型油菜/萌发期/耐盐性/化学诱变/EMS诱变剂Key words
Brassica napus L./germination stage/salt-tolerance/chemical mutagenesis/EMS mutagen分类
农业科技引用本文复制引用
何棱,曾长立,南建军,曹永胜,沈金雄,傅廷栋,万何平..油菜EMS诱变群体萌发期耐盐突变体筛选与鉴定[J].江汉大学学报（自然科学版）,2025,53(5):16-22,7.基金项目
国家生物育种重大项目(2022ZD04010) （2022ZD04010）
国家自然科学基金项目(U22A20469) （U22A20469）
