江汉大学学报（自然科学版）2025，Vol.53Issue(5)：61-69,9.DOI:10.16389/j.cnki.cn42-1737/n.2025.05.007
橙皮素对水浸束缚应激小鼠空肠损伤的保护作用研究
Protective Effect of Hesperetin on Jejunal Injury in Mice Subjected to Water Immersion Restraint Stress
摘要
Abstract
Objective To investigate the protective effects of hesperetin on jejunal injury in mice subjected to water immersion restraint stress(WIRS).Methods A WIRS model was established,with mice randomly assigned to four groups:control,model,low-dose hesperetin(50 mg/kg),and high-dose hesperetin(100 mg/kg).Mice in the hesperetin groups were administered their respective doses for 7 consecutive days before undergoing the stress protocol.The protective effects of hesperetin were assessed by measuring serum levels of AST and ALT,tissue MDA content,and T-SOD activity,as well as serum TNF-α and IL-1β levels via ELISA.Histopathological analysis of the jejunum tissue was performed using HE staining,and villus height,crypt depth,and the villus height to crypt depth ratio were quantified.Results HE staining revealed that both low-and high-dose hesperetin groups significantly reduced serum AST and ALT levels,decreased MDA content,enhanced T-SOD activity,and suppressed TNF-α and IL-1β expression compared to the model group.Furthermore,hesperetin treatment resulted in increased villus height,reduced crypt depth,and an improved villus height to crypt depth ratio.HE staining indicated that hesperetin significantly alleviated jejunal tissue injury in stressed mice.Conclusion Hesperetin exerts significant protective effects against jejunal injury induced by WIRS in mice.This protective action is likely mediated by its antioxidant properties,anti-inflammatory effects,and ability to promote intestinal tissue repair.关键词
橙皮素/水浸束缚应激/空肠损伤/抗氧化作用/炎症反应Key words
hesperetin/water immersion restraint stress/jejunal injury/antioxidant effect/inflammatory response分类
医药卫生引用本文复制引用
何佩宜,郭漫儿,郑仕伟,臧林泉,祝曙光..橙皮素对水浸束缚应激小鼠空肠损伤的保护作用研究[J].江汉大学学报（自然科学版）,2025,53(5):61-69,9.基金项目
广东省自然科学基金项目(2017A030313856) （2017A030313856）
