检验医学与临床2025，Vol.22Issue(19)：2599-2605,7.DOI:10.3969/j.issn.1672-9455.2025.19.002
miR-103a-3p、CDCA5 mRNA在肺癌组织中的水平及临床意义
The levels and clinical significance of miR-103a-3p and CDCA5 mRNA in lung cancer tissues
摘要
Abstract
Objective To investigate the levels and clinical significance of miR-103a-3p and cell division cy-cle related protein 5(CDCA5)mRNA in lung cancer tissues.Methods A total of 90 patients with lung cancer admitted to the hospital from October 2016 to September 2019 were selected as the research objects.The mR-NA levels of miR-103a-3p and CDCA5 in lung cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction.Pearson correlation was used to ana-lyze the correlation between miR-103a-3p levels and CDCA5 mRNA levels in lung cancer tissues.StarBase website was used to analyze the targeting relationship between miR-103a-3p and CDCA5 mRNA.Survival a-nalysis was performed using the K-M curve.Multivariate Cox regression analysis was used to analyze the in-fluencing factors of 5-year mortality in patients with lung cancer after surgery.Results The level of miR-103a-3p in lung cancer tissues was lower than that in adjacent tissues,Pearson correlation analysis showed that the level of CDCA5 mRNA was higher than that in adjacent tissues,and the differences were statistically sig-nificant(P＜0.05).miR-103a-3p and CDCA5 mRNA had a targeting relationship in lung cancer tissues,Pear-son correlation analysis showed that the levels of miR-103a-3p and CDCA5 mRNA in lung cancer tissues were negatively correlated(r=—0.458,P＜0.001).The proportions of low expression of miR-103a-3p and high expression of CDCA5 mRNA in lung cancer tissues of patients with TNM stage Ⅲ.poorly differentiated,dis-tant metastasis,and pleural invasion were lower than those of patients with TNM stage Ⅰ—Ⅱ,moderately and highly differentiated,no distant metastasis,and no pleural invasion,and the differences were statistically significant(P＜0.05).During the 5-year follow-up,58 patients survived and 32 died.The level of miR-103a-3p in lung cancer tissues of survival lung cancer patients was higher than that in dead lung cancer patients,and the level of CDCA5 mRNA was lower than that in dead lung cancer patients,and the differences were statisti-cally significant(P＜0.05).The 5-year survival rate of lung cancer tissues of lung cancer patients with high expression of miR-103a-3p was 77.27％,which was higher than 52.17％of lung cancer patients with low ex-pression,and the difference was statistically significant(x2=6.183,P=0.013).The 5-year survival rate of in lung cancer tissues of lung cancer patients with high CDCA5 mRNA expression was 48.94％,which was lower than 81.40％of in lung cancer tissues of lung cancer patients with low CDCA5 mRNA expression,and the difference was statistically significant(x2=10.325,P=0.001).The survival curve of lung cancer patients with high miR-103a-3p expression was higher than that of patients with low miR-103a-3p expression(Log-rank x2=3.762,P＜0.001).The survival curve of lung cancer tissues of lung cancer patients with CDCA5 mRNA high expression was lower than that of CDCA5 mRNA low expression lung cancer patients(Log-rank x2=3.561,P＜0.001).Cox regression analysis showed that the increased level of CDCA5 mRNA was an in-dependent risk factor for 5-year death in lung cancer tissues of patients with lung cancer after surgery(P＜0.05).The increased level of miR-103a-3p was an independent protective factor for 5-year death in patients with lung cancer after surgery(P＜0.05).Conclusion The level of miR-103a-3p is lower and CDCA5 mRNA level is higher in lung cancer tissues.The levels of miR-103a-3p and CDCA5 mRNA in lung cancer tissues were negatively correlated,that is,the lower the level of miR-103a-3p,the higher the CDCA5 mRNA level,and the higher the risk of death in patients with lung cancer after surgery.关键词
肺癌/miR-103a-3p/细胞分裂周期相关蛋白5/病理特征/临床意义Key words
lung cancer/miR-103a-3p/cell division cycle-related protein 5/pathological features/clinical significance分类
医药卫生引用本文复制引用
辛欢,王裕虎..miR-103a-3p、CDCA5 mRNA在肺癌组织中的水平及临床意义[J].检验医学与临床,2025,22(19):2599-2605,7.基金项目
陕西省咸阳市重点研发计划(S2023-ZDYF-SF-2004). （S2023-ZDYF-SF-2004）
