检验医学与临床2025，Vol.22Issue(19)：2671-2677,7.DOI:10.3969/j.issn.1672-9455.2025.19.015
血清miR-155-3p和miR-183-3p水平与腰椎间盘退变程度的关系
Relationship between serum miR-155-3p and miR-183-3p levels with the Degree of lumbar intervertebral disc degeneration
摘要
Abstract
Objective To investigate the relationship between serum miR-155-3p,miR-183-3p levels and the degree of lumbar intervertebral disc degeneration(LDD).Methods A total of 132 patients with LDD ad-mitted to the hospital from June 2021 to June 2023 were selected as the study group.A total of 40 LDD pa-tients with Pfirrmann grade Ⅲ were included in the mild group,and a total of 42 LDD patients with Pfirrmann grade Ⅳ were included in the moderate group,and 50 LDD patients with Pfirrmann grade V were included in the severe group.At the same time,132 healthy people were selected as the control group.The serum levels of miR-155-3p and miR-183-3p were detected by real-time fluorescent quantitative reverse transcription polymer-ase chain reaction.The serum levels of interleukin(IL)-6 and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay.Spearman correlation analysis was used to analyze the correlation be-tween serum miR-155-3p,miR-183-3p levels and the degree of LDD in LDD patients.Pearson correlation anal-ysis was used to analyze the correlation between serum miR-155-3p,miR-183-3p levels and serum IL-6,TNF-α levels,Japanese Orthopaedic Association evaluation treatment(JOA)score,visual analogue scale(VAS)score in LDD patients.Multivariate Logistic regression was used to analyze the influencing factors of severe degeneration in LDD patients.The receiver operating characteristic(ROC)curve was used to analyze the diag-nostic value of serum miR-155-3p and miR-183-3p for severe degeneration in LDD patients.Results The level of serum miR-155-3p in the study group was lower than that in the control group,and the level of serum miR-183-3p was higher than that in the control group,and the differences were statistically significant(P＜0.05).The serum levels of miR-183-3p,IL-6,TNF-α and VAS score in the mild group,moderate group and severe group were increased in turn,and the serum level of miR-155-3p and JOA score were decreased in turn,and the differences were statistically significant(P＜0.05).Pearson correlation analysis showed that serum miR-155-3p level was negatively correlated with serum IL-6 and TNF-α levels and VAS score(P＜0.05),and posi-tively correlated with JOA score in LDD patients(P＜0.05).Serum miR-183-3p level was positively correla-ted with serum IL-6 and TNF-α levels and VAS score(P＜0.05),and negatively correlated with JOA score in LDD patients(P＜0.05).Spearman correlation analysis showed that serum miR-155-3p level was negatively correlated with the degree of LDD in LDD patients(P＜0.05),and serum miR-183-3p was positively correla-ted with the degree of LDD in LDD patients(P＜0.05).Multivariate Logistic regression analysis showed that the increased levels of serum miR-183-3p,IL-6 and TNF-α were all risk factors for severe degeneration in LDD patients(P＜0.05).Increased serum miR-155-3p level was a protective factor for severe degeneration in LDD patients(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of serum miR-155-3p,miR-183-3p alone and their combination for the diagnosis of severe degeneration in patients with LDD were 0.837,0.812 and 0.876 respectively,and the AUC of the combination of miR-155-3p and miR-183-3p in the diagnosis of severe degeneration in LDD patients was greater than the two alone,and the differences were sta-tistically significant(Z=3.616,2.797,P＜0.001,=0.005).Conclusion Serum miR-155-3p and miR-183-3p levels were closely related to the degree of LDD.关键词
腰椎间盘退变/退变程度/miR-155-3p/miR-183-3p/相关性Key words
lumbar disc degeneration/degree of degeneration/miR-155-3p/miR-183-3p/correla-tion分类
医药卫生引用本文复制引用
师艳艳,张凯,杨武燕,孙惠,张金盼,李建伟..血清miR-155-3p和miR-183-3p水平与腰椎间盘退变程度的关系[J].检验医学与临床,2025,22(19):2671-2677,7.基金项目
河北省医学科学研究课题计划(20220572). （20220572）
