临床口腔医学杂志2025，Vol.41Issue(10)：591-596,6.DOI:10.3969/j.issn.1003-1634.2025.10.004
lncRNA-TNFRSF13C对脂多糖处理的牙周膜细胞凋亡周期及对miR-520的调控作用
The effect of lncRNA-TNFRSF13C on the apoptotic cycle of periodontal cells treated with lipopolysaccharide and the regulation of miR-520
摘要
Abstract
Objective:To explore the effect of lncRNA-TNFRSF13C on the apoptosis cycle of periodontal ligament(PDL)cells treated with lipopolysaccharide(LPS)and its regulation on miR-520.Methods:Periodontal ligament cells were taken,and lncRNA-TNFRSF13C and miR-520-related plasmids were transfected into the cells.The expression levels of lncRNA-TNFRSF13C and miR-520 were detected by Real-time PCR,and cell apoptosis and cell cycle were detected by flow cytometry.The dual-luciferase reporter assay confirmed the interaction between lncRNA-TNFRSF13C and miR-520.Results:Compared with the PM group,The expression level of lncRNA-TNFRSF13C mRNA in the LP group was significantly in-creased(P<0.05),and the expression level of miR-520 mRNA was significantly decreased(P<0.05).Compared with the PM group,the apoptosis rate and the proportion of G1 phase in the LP group were significantly increased(P<0.05),while the proportions of S phase and G2 phase were significantly decreased(P<0.05).Compared with the LP group,there was no sig-nificant statistical difference in the apoptosis rate and cycle in the TN and MN groups(P>0.05).Compared with the TN and MN groups,the apoptosis rate and the proportion of the G1 phase in the TM group and the MI group were significantly in-creased(P<0.05),while the proportions of the S phase and the G2 phase were significantly decreased(P<0.05).The apop-tosis rate and the proportion of the G1 phase in the TI group and the MM group were significantly decreased(P<0.05),while the proportions of the S phase and the G2 phase were significantly increased(P<0.05).Compared with the TI group,there was no significant statistical difference in the apoptosis rate and the proportion of G1 phase in the MM group(P>0.05).Com-pared with the MM group,the apoptosis rate and the proportion of G1 phase in the IM group were significantly decreased(P<0.05),and the proportions of S phase and G2 phase were significantly increased(P<0.05).The results of the dual-luciferase report showed that transfection of lncRNA-TNFRSF13C could significantly reduce the luciferase activity of miR-520-3′-UTR-WT(P<0.05),but had no significant effect on the mutant gene(P>0.05).Conclusion:Inhibiting lncRNA-TNFRSF13C can effectively inhibit apoptosis of PDL cells and weaken the inhibitory effect of LPS on cell cycle progression in PDL cells,which may be related to targeted activation of miR-520.关键词
lncRNA-TNFRSF13C/miR-520/脂多糖/牙周膜细胞/凋亡周期Key words
lncRNA-TNFRSF13C/miR-520/Lipopolysaccharide/Periodontal membrane cells/Apoptotic cycle分类
医药卫生引用本文复制引用
张雪,任燕,白静,李月辉..lncRNA-TNFRSF13C对脂多糖处理的牙周膜细胞凋亡周期及对miR-520的调控作用[J].临床口腔医学杂志,2025,41(10):591-596,6.基金项目
河北省医学科学基金资助项目(编号:20230240) （编号:20230240）
