临床与实验病理学杂志2025，Vol.41Issue(9)：1134-1141,1148,9.DOI:10.13315/j.cnki.cjcep.2025.09.003
胶质瘤细胞分泌Prg4诱导巨噬细胞选择性活化关键调控分子Dicer表达
Glioma cell-secreted Prg4 induces the expression of macrophage Dicer,a key reg-ulatory molecule for macrophage alternative activation
摘要
Abstract
Purpose To explore the key molecules mechanisms underlying the selective activation of macrophage and the regulation of Dicer expression induced by glioblastoma(GBM)cells,as well as its prognostic significance.Methods Glioblastoma conditional medium(GCM)was fractionated by molecular weight using ultrafiltration.Specif-ic molecular weight components of GCM that upregulate Dicer expression in mouse bone marrow derived macrophages(BMDMs)were identified.Secreted proteins were identified by mass spectrometry(MS).The correlation between candidate proteins and GBM prognosis was analyzed using the TCGA and CGGA database.In vitro experiments of the candidate proteins on Dicer expression in BMDMs were further carried out.Results GCM components with a molecu-lar weight of＞50 kDa significantly upregulated Dicer expression in BMDMs.MS identified five key secreted proteins:Prg4,Psap,Hexa,Aebp1,and Itih2.High expression of Prg4 was significantly positively correlated with poor progno-sis in GBM patients(P＜0.001)and was associated with the expression of selective macrophage activation markers.Recombinant Prg4 protein stimulated BMDMs and induced Dicer expression in mouse BMDMs.Conclusion This study reveals that glioma cells induce Dicer expression in macrophages by secreting Prg4,providing a theoretical basis for GBM therapeutic strategies targeting the Prg4-Dicer axis.关键词
胶质母细胞瘤/巨噬细胞/Dicer/Prg4Key words
glioblastoma/macrophage/Dicer/Prg4分类
医药卫生引用本文复制引用
李舒宜,钟京含,刘宇奇,罗敏,平轶芳,卞修武..胶质瘤细胞分泌Prg4诱导巨噬细胞选择性活化关键调控分子Dicer表达[J].临床与实验病理学杂志,2025,41(9):1134-1141,1148,9.基金项目
重庆市技术创新与应用发展专项重大项目(CSTB2022TIAD-STX0010)、西藏自治区自然科学基金(XZ202401ZR0116) Science and Technology Innovation Key R&D Program of Chongqing(CSTB2022TIAD-STX0010) （CSTB2022TIAD-STX0010）
Science and Technology Innovation Project of Xizang Science and Technology Commission(XZ202401ZR0116) （XZ202401ZR0116）
