临床与实验病理学杂志2025，Vol.41Issue(9)：1149-1155,7.DOI:10.13315/j.cnki.cjcep.2025.09.005
胶质瘤中ELAVL家族的表达及临床意义
Expression of ELAVL family in glioma and their clinicopathological significance
摘要
Abstract
Purpose To investigate the expression and clinical significance of the embryonic lethal abnormal vi-sion-like(ELAVL)family in glioma.Methods Pan-cancer and glioma-specific analyses of mRNA expression profiles of the ELAVL family were analyzed using the TCGA and GTEx databases.The association between ELAVL family ex-pression and survival of glioma patients was evaluated via the gene expression profiling interactive analysis 2(GEPIA2)database.The expression level of ELAVL2 protein in human glioma tissues and non-tumor control brain tissues was ver-ified by immunohistochemistry,and the relationship between its expression and prognosis was analyzed based on the fol-low-up data of patients.Western blot was performed to assess ELAVL2 protein levels in human immortalized astrocytes of UC2 and seven glioma cell lines.Overexpression of ELAVL2 in glioma cells was achieved to evaluate its impact on cell proliferation using in vitro assays.Results Compared to normal tissues,the ELAVL family exhibited distinct ex-pression patterns across various cancers.In glioma,ELAVL1 was significantly upregulated,while ELAVL2,ELAVL3 and ELAVL4 were markedly downregulated.Survival analysis revealed that low ELAVL2 expression was an independent prognostic factor for poor survival in glioma patients(P＜0.05).Immunohistochemistry confirmed that the expression of ELAVL2 decreased with the increase of glioma grade,and its low expression indicated a poor prognosis for patients(P＜0.001).Overexpression of ELAVL2 inhibited glioma cell proliferation in vitro(P＜0.001),suggesting its tumor-suppressive role.Conclusion The ELAVL family members play a critical role in glioma progression.ELAVL2 downregulation serves as a marker for adverse clinical outcomes and represents a potential therapeutic target for glioma therapy.关键词
胶质瘤/ELAVL家族/预后/增殖Key words
gliomas/ELAVL family/prognosis/proliferation分类
医药卫生引用本文复制引用
杨晴晴,罗文君,孙翠云,于士柱,周雪霞..胶质瘤中ELAVL家族的表达及临床意义[J].临床与实验病理学杂志,2025,41(9):1149-1155,7.基金项目
国家自然科学基金(82172901、82472643)、天津医科大学总医院优秀青年基金(22ZYYYQ01) National Natural Science Foundation of China(82172901,82472643) （82172901、82472643）
Tianjin Medical University General Hospital Outstanding Youth Fund(22ZYYYQ01) （22ZYYYQ01）
