现代畜牧兽医Issue(9)：29-35,7.DOI:10.20154/j.cnki.issn1672-9692.2025.09.005
玉米蛋白粉主料菌酶协同发酵功能饲料的制备研究
Research on the preparation of functional feed by co-fermentation of corn protein powder as the main material with bacteria and enzymes
摘要
Abstract
The experiment adopted the co-fermentation technology of bacteria and enzymes to prepare the main material fermentation functional feed of corn protein powder,aiming to improve the bioavailability of corn protein powder.The proportion of experimental materials was corn protein meal∶soybean meal∶corn germ meal=5∶3∶2.First,the proportion of mixed bacteria inoculation was determined through orthogonal experiments.Then,the fermentation conditions were optimized through single-factor experiments and response surface experiments,and the contents of the main nutrients in the feed before and after fermentation were compared.The results showed that the mixed inoculation ratio was Bacillus subtilis YY18∶Saccharomyces cerevisiae YY21∶Lactobacillus plantarum YY19=4∶1∶1.The optimal fermentation conditions are as follows:The total inoculation amount of mixed bacteria is 11%,the addition amount of acidic protease is 80 U/g,the addition amount of lysine mother liquor is 5%,the fermentation temperature is 31℃,and the fermentation time is 3 days.Under these conditions,the contents of soluble protein and small peptides and the number of viable bacteria in the fermented feed prepared were 148.46 mg/g,96.38 mg/g,and 1.20×109 CFU/g,respectively.Compared with unfermented feed,the content of crude fiber in the fermented feed was significantly reduced(P<0.05),while the contents of soluble protein and small peptides increased by 2.45 times and 2.74 times respectively(P<0.05).Research shows that the co-fermentation technology of bacteria and enzymes can effectively enhance the bioavailability and nutritional value of feed raw materials,laying a theoretical foundation for the efficient utilization of corn protein powder in the feed field.关键词
玉米蛋白粉/菌酶协同发酵/氮代谢/小肽Key words
Corn protein powder/Co-fermentation of bacteria and enzymes/Nitrogen metabolism/Small peptides分类
农业科技引用本文复制引用
邓祥卿,刘晓兰,李冠龙,王富赟,郑喜群..玉米蛋白粉主料菌酶协同发酵功能饲料的制备研究[J].现代畜牧兽医,2025,(9):29-35,7.基金项目
中央引导地方科技发展专项(ZY23QY06) （ZY23QY06）
黑龙江省"双一流"建设学科协同创新成果建设项目(LJGXCG2023-104、LJGXCG2023-055) （LJGXCG2023-104、LJGXCG2023-055）
