南京中医药大学学报2025，Vol.41Issue(9)：1189-1197,9.DOI:10.14148/j.issn.1672-0482.2025.1189
基于网络药理学研究滋阴明目方抑制激光诱发小鼠脉络膜新生血管的作用及机制
Study on the Effect and Mechanism of Ziyin Mingmu Formula in Inhibiting Laser-Induced Choroidal Neovascularization in Mice Based on Network Pharmacology
摘要
Abstract
OBJECTIVE To investigate the effect and potential mechanism of Ziyin Mingmu Formula in treating age-related mac-ular degeneration(AMD)by combining network pharmacology with animal model validation.METHODS Active ingredients of Ziyin Mingmu Formula were obtained from the TCMSP and BATMAN databases,and their targets were searched using the PharmMapper da-tabase.AMD disease targets were identified using the GeneCards,DrugBank,OMIM,and TTD databases.Venny analysis was per-formed to identify the intersection of active ingredient and disease targets.A protein-protein interaction(PPI)network was constructed using the String database,and core targets were screened using Cytoscape 3.9.0 software.KEGG pathway enrichment analysis was per-formed using the DAVID database.Molecular docking of key active ingredients with core targets was performed using Autodock software.A laser-induced mouse choroidal neovascularization(CNV)model was used.Optical coherence tomography angiography(OCTA)was used to assess CNV area in vivo,immunofluorescence staining was used to assess CNV area on choroidal flat mounts,and Western blot analysis was used to examine the expression of proteins involved in the vascular endothelial growth factor(VEGF)pathway.RESULTS Network pharmacology analysis identified 221 active ingredients in the Ziyin Mingmu Formula.PPI analysis i-dentified 29 core targets,including SRC,protein kinase B(AKT1),mitogen-activated protein kinase 1(MAPK1),and heat shock protein 90α family class A member 1(HSP90AA1).KEGG analysis revealed that the VEGF signaling pathway was the most highly en-riched.Molecular docking revealed that the core targets SRC,AKT1,MAPK1,and HSP90AA1 had good binding affinity with the main active ingredients,diosmetin,catechin,vestitol,and licochalcone A.Animal experiments showed that the Ziyin Mingmu Formula significantly reduced CNV area in model mice,downregulated VEGF protein expression,decreased VEGFR2,p38,and ERK1/2 pro-tein phosphorylation levels,and inhibited the VEGF signaling pathway.CONCLUSION Ziyin Mingmu Formula may inhibit CNV for-mation by regulating the VEGF signaling pathway.关键词
滋阴明目方/CNV/年龄相关性黄斑变性/网络药理学/VEGF信号通路Key words
Ziyin Mingmu Formula/CNV/age-related macular degeneration/network pharmacology/VEGF signaling pathway分类
医药卫生引用本文复制引用
周欣,熊彩建,杨宁,金青子,陶康,袁媛,施炜,冯哲,徐新荣..基于网络药理学研究滋阴明目方抑制激光诱发小鼠脉络膜新生血管的作用及机制[J].南京中医药大学学报,2025,41(9):1189-1197,9.基金项目
国家自然科学基金面上项目(82374214) （82374214）
中国中医科学院学部委员学术传承与传播专项(CI2022E028XB) （CI2022E028XB）
江苏省(社会发展)重点研发计划(BE2019723) （社会发展）
