南京中医药大学学报2025，Vol.41Issue(9)：1198-1212,15.DOI:10.14148/j.issn.1672-0482.2025.1198
UPLC-Q-TOF-MS/MS和GC-MS法分析银翘清热片化学成分
Analysis of Chemical Components of Yin-Qiao-Qing-Re Tablets by UPLC-Q-TOF-MS/MS and GC-MS
摘要
Abstract
OBJECTIVE The non-volatile and volatile chemical components in Yin-Qiao-Qing-Re Tablets were analyzed sepa-rately using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)and Gas Chromatography Mass Spectrometry(GC-MS).METHODS The non-volatile components were analyzed using a Waters ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7 μm),with a mobile phase consisting of 0.1％formic acid aqueous solution(A)and acetonitrile(B)for gradient elution,a flow rate of 0.35 mL·min-1,an injection volume of 5 μL,and a column temperature of 30 ℃;the volatile components were analyzed using an Agilent SH-I-5MS column(5％Phenyl Methyl Silox,30 m×250 μm,0.25 μm);the procedure was temperature-programmed,with an injection volume of 1 μL,a split ratio of 10∶1,a flow rate of 1.0 mL·min-1,and an inlet temperature of 200 ℃.RESULTS A total of 134 non-volatile chemical components and 23 volatile components were analyzed and identified from Yin-Qiao-Qing-Re Tablets,among which 49 compounds were confirmed through comparison with reference stand-ards.The non-volatile components mainly include 27 flavonoids,21 organic acids,15 lignans,14 iridoids,12 phenylethanoid glyco-sides,11 saponins,10 alkaloids,5 terpenes,4 amino acids,3 phenylpropanoids,3 nucleosides,3 xanthones,3 phenolic glycosides,2 chromones and 1 carbohydrate.The volatile components mainly include 11 monoterpenes,5 alcohols and phenols,3 alkenes,2 ke-tones,1 ester,and 1 hydrocarbon.CONCLUSION This study rapidly identifies the chemical components of Yin-Qiao-Qing-Re Tablets,laying a preliminary foundation for research on the pharmacodynamic substances of Yin-Qiao-Qing-Re Tablets and the im-provement of quality control standards.关键词
银翘清热片/化学成分/UPLC-Q-TOF-MS/MS/GC-MSKey words
Yin-Qiao-Qing-Re Tablets/chemical components/UPLC-Q-TOF-MS/MS/GC-MS分类
医药卫生引用本文复制引用
蔡泽荣,胡玉梅,刘文君,汪树林,孔心雨,杨一帆,钱梦雨,曹亮,王振中..UPLC-Q-TOF-MS/MS和GC-MS法分析银翘清热片化学成分[J].南京中医药大学学报,2025,41(9):1198-1212,15.基金项目
江苏省工业和信息产业转型升级专项(JITC-2000AX0676) （JITC-2000AX0676）
连云港市博士后科研资助项目(LYG20220024) （LYG20220024）
