南京中医药大学学报2025，Vol.41Issue(9)：1213-1223,11.DOI:10.14148/j.issn.1672-0482.2025.1213
不同干燥工艺的当归补血颗粒指纹图谱的建立及抗氧化作用的谱效关系研究
Establishment of Fingerprints of Danggui Buxue Granules with Different Drying Processes and Study of the Spectrum-Effect Relationship of Antioxidant Activity
摘要
Abstract
OBJECTIVE To establish HPLC fingerprints of three drying processes(Atmospheric pressure drying,Decompression drying,Freeze-drying)of Danggui Buxue granules,and combine them with antioxidant tests to determine the optimal drying process and main active components of Danggui Buxue granules.METHODS The fingerprints of multiple batches of Danggui Buxue granules were established by HPLC;the"Fingerprint Similarity Evaluation System of Traditional Chinese Medicine Chromatogram"was used to evaluate the similarity;Hierarchical Cluster Analysis(HCA)and Principal Component Analysis(PCA)were used to characterize the different drying processes of Danggui Buxue granules;evaluate the antioxidant activity of Danggui Buxue granules in different drying processes using 1,1-diphenyl-2-picrylhydrazy(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)free radical scavenging method;the spectrum-effect relationship between Danggui Buxue granules fingerprint and antioxi-dant activity was analyzed by grey correlation degree and Pearson,Spearman,Kendall's tau-b correlation analysis methods.RE-SULTS The results of fingerprint showed that there were 16 common peaks in 30 batches of Danggui Buxue granules,and 6 of them were identified by comparison.The results of Cluster Analysis and Principal Component Analysis showed that 30 batches of Danggui buxue granules were divided into 3 categories,and the difference between the groups of atmospheric pressure drying was the least.The results of oxidation test showed that different drying processes of Danggui Buxue granules had good antioxidant activity,and the atmos-pheric pressure drying had the lowest IC50 and the strongest antioxidant activity.Finally,combining the results of gray correlation anal-ysis and correlation analysis,the compounds F2,F10,F13(calycosin),F15(formononetin),F16 might be important characteristic peaks reflecting the antioxidant activity of Danggui Buxue granules.CONCLUSION Compared with other drying processes,atmos-pheric pressure drying has higher batch consistency and stronger antioxidant activity,and can be used as the preferred drying process for Danggui Buxue granules,and components 2,10,13,15 and 16 are the main active ingredients for Danggui Buxue granules to exert antioxidant effects.关键词
当归补血颗粒/不同干燥工艺/指纹图谱/抗氧化/谱效关系Key words
Danggui Buxue granules/different drying processes/fingerprint/antioxidant/spectrum-effect relationship分类
医药卫生引用本文复制引用
陈盼,高武锋,张志同,关欢欢,白雷,刘文健,陈丽,袁冬平,严国俊..不同干燥工艺的当归补血颗粒指纹图谱的建立及抗氧化作用的谱效关系研究[J].南京中医药大学学报,2025,41(9):1213-1223,11.基金项目
中药学一流学科前沿探索性交叉科学研究"揭榜挂帅"项目(ZYXJC2024-009) （ZYXJC2024-009）
中药制药过程控制与智能制造技术全国重点实验室创新项目(NZYSKL240108) （NZYSKL240108）
国家自然科学基金面上项目(81773910,82074004) （81773910,82074004）
