北方农业学报2025，Vol.53Issue(3)：60-75,16.DOI:10.12190/j.issn.2096-1197.2025.03.07
嫩江市大豆田反枝苋和藜对氟磺胺草醚的抗性
Resistance of Amaranthus retroflexus L.and Chenopodium album L.to fomesafen in soybean fields of Nenjiang City
摘要
Abstract
[Objective]To determine the resistance levels and distribution of the broadleaf weeds Amaranthus retroflexus L.and Chenopodium album L.to fomesafen in soybean fields of Nenjiang City,Heilongjiang Province.[Methods]Seeds of A.retroflexus L.and C.album L.were collected from 10 towns,including Nenjiang Town,Haijiang Town,Qianjin Town etc.The resistance levels of weeds of A.retroflexus L.and C.album L.to fomesafen were determined using a whole-plant bioassay.The mechanisms underlying resistance in A.retroflexus L.were investigated through sequence amplification and alignment of the target gene PPX2 and other related methods.[Results]After treatment with different doses of fomesafen,the GR50 values for 30 collected A.retroflexus L.populations ranged from 8.70 to 814.77 g a.i./hm2,with resistance indices(RI)of 17.8 to 93.6.Fourteen populations exhibited low-level resistance,primarily collected from Lianxing Township,Yilaha Town,and Taxi Township;ten populations showed moderate-level resistance,mainly from Shuangshan Town,Changfu Town,and Keluo Town;and two populations displayed high-level resistance,primarily from Haijiang Town.Target gene detection revealed that all 26 resistant A.retroflexus L.populations had an Arg-128Gly mutation in the PPX2 gene.A single resistant population of A.retroflexus L.R was selected and treated with P450 and GST inhibitors,resulting in a significant reduction of RI,indicating the presence of non-target-site resistance.After treatment with varying doses of fomesafen,the GR50 values of 26 collected C.album L.populations ranged from 51.54 to 497.10 g a.i./hm2,with RI of 1.0 to 9.5.Ten populations exhibited low-level resistance,six showed moderate-level resistance,and no high-level resistant populations to fomesafen were identified.Resistant C.album L.populations to fomesafen were primarily collected from Linjiang Township,Changfu Town,and Keluo Town.[Conclusion]A.retroflexus L.and C.album L.in Nenjiang City,Heilongjiang Province,have developed different levels of resistance to fomesafen,with A.retroflexus L.exhibiting both target-site resistance and non-target-site resistance to fomesafen.关键词
嫩江市/大豆田/反枝苋/藜/氟磺胺草醚/抗性Key words
Nenjiang City/Soybean field/Amaranthus retroflexus L./Chenopodium album L./Fomesafen/Resistance分类
农业科技引用本文复制引用
郭小桐,郭玉莲,王宇,罗婵,丛克强..嫩江市大豆田反枝苋和藜对氟磺胺草醚的抗性[J].北方农业学报,2025,53(3):60-75,16.基金项目
黑龙江省创新跨越工程农业关键技术科技创新重点攻关项目(CX23GG14) （CX23GG14）
