北方农业学报2025，Vol.53Issue(3)：87-92,6.DOI:10.12190/j.issn.2096-1197.2025.03.09
蝴蝶兰对低温胁迫的生理响应及耐寒性评价
Physiological responses and cold tolerance evaluation of Phalaenopsis under low-temperature stress
摘要
Abstract
[Objective]To investigate the physiological responses and differences in cold tolerance among Phalaenopsis under low-temperature stress,and provide support for improving cold-tolerance traits and breeding new cold-tolerant cultivars.[Methods]Three Phalaenopsis cultivars(P.Big Chili,P.Little Gem Stripes,and P.Wedding Promenade)were used as experimental materials and subjected to low-temperature stress(10,5,0℃)and non-stressed(CK,25℃)conditions.The activities of peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),and the contents of proline(Pro)and malondialdehyde(MDA)in detached leaves under these conditions were measured.The cold tolerance was comprehensively evaluated using the membership function method.[Results]Under low-temperature stress,the physiological responses of the tested Phalaenopsis cultivars differed significantly from those under non-stressed conditions.In the P.Wedding Promenade cultivar,the activities of POD,CAT,SOD,and the contents of Pro and MDA exhibited a gradual decline with decreasing temperature,peaking at 10℃with values of 220.73 U/(g·min),44.00 U/(g·min),261.19 U/g,and 289.31 μg/g,44.00 μmol/g,respectively.For the P.Big Chili and P.Little Gem Stripes cultivars,the activities of POD,CAT,SOD,and the contents of Pro and MDA exhibited an initial increase followed by a decrease,all peaking at 5℃.The comprehensive membership function values for P.Wedding Promenade,P.Big Chili,and P.Little Gem Stripes were 0.540,0.447,and 0.357,respectively.[Conclusion]The three Phalaenopsis cultivars exhibited different levels of tolerance and physiological responses to low-temperature stress,with cold tolerance ranked as P.Wedding Promenade>P.Big Chili>P.Little Gem Stripes,which is consistent with their performance in actual cultivation.关键词
蝴蝶兰/低温胁迫/生理响应/耐寒性/隶属函数法Key words
Phalaenopsis/Low-temperature stress/Physiological response/Cold tolerance/Membership function method分类
农业科技引用本文复制引用
王世尧,杨书才,蒋拴丽,王瑞华,张果,王俊,冯建,杨录军,赵玉安..蝴蝶兰对低温胁迫的生理响应及耐寒性评价[J].北方农业学报,2025,53(3):87-92,6.基金项目
河南省重点研发与推广专项(科技攻关,232102111101) （科技攻关,232102111101）
河南省林木种质资源建设项目(豫财环资[2023]60号,豫财环资[2024]47号) （豫财环资[2023]60号,豫财环资[2024]47号）
