北方农业学报2025，Vol.53Issue(3)：93-103,11.DOI:10.12190/j.issn.2096-1197.2025.03.10
紫苏叶总黄酮提取工艺优化及抗菌抗氧化研究
Study on the optimization of extraction process and antibacterial and antioxidant activities of perilla leaf total flavonoids
摘要
Abstract
[Objective]To determine the optimal conditions for ultrasound-assisted ethanol extraction of perilla leaf total flavonoids and to evaluate their antibacterial and antioxidant activities.[Methods]After determining the optimal conditions for each factor through single-factor experiments,response surface methodology was employed to design the extraction process optimization experiment,and the optimal extraction conditions for perilla leaf total flavonoids were determined using Design-Expert 13.0.The antibacterial capacity was assessed using the inhibitory effect on Staphylococcus aureus as an indicator,while antioxidant activity was evaluated through DPPH and ABTS free radical scavenging assays.[Results]Under different factor levels,the extraction yields of perilla leaf total flavonoids reached the highest values of 2.72%,3.20%,2.76%,and 3.02%,respectively,with the ethanol volume fraction of 35%,liquid-to-solid ratio of 15∶1(mL∶g),ultrasonic power of 300 W,and extraction time of 30 min.After optimization using response surface methodology,the extraction yield of perilla leaf total flavonoids reached a maximum of(3.375±0.098)%under the conditions of 36%ethanol volume fraction,liquid-to-solid ratio of 17∶1(mL∶g),ultrasonic power of 303 W,and extraction time of 33 min.The obtained perilla leaf total flavonoids extract exhibited a significant inhibition zone against Staphylococcus aureus,with maximum DPPH and ABTS free radical scavenging rates of 97.86%and 95.79%,respectively.[Conclusion]The optimal extraction conditions for perilla leaf total flavonoids are an ethanol volume fraction of 36%,liquid-to-solid ratio of 17∶1(mL∶g),ultrasonic power of 303 W,and extraction time of 33 min.The perilla leaf total flavonoids demonstrate significant inhibitory effects against Staphylococcus aureus and strong free radical scavenging capacity.关键词
紫苏叶总黄酮/响应面分析法/提取工艺/抗菌/抗氧化Key words
Perilla leaf total flavonoids/Response surface methodology/Extraction process/Antibacterial activity/Antioxidant activity分类
农业科技引用本文复制引用
许梦璐,许景开,邱磊,崔含章,张开涛,陈飞雨,罗进城..紫苏叶总黄酮提取工艺优化及抗菌抗氧化研究[J].北方农业学报,2025,53(3):93-103,11.基金项目
佳木斯大学2024年省级大学生创新创业计划项目(S202410222116) （S202410222116）
