山东科学2025，Vol.38Issue(5)：10-21,12.DOI:10.3976/j.issn.1002-4026.20240113
响应面法优化酶-水连续提取西洋参须根人参皂苷
Optimization of enzyme-water continuous extraction of ginsenosides from fibrous roots of American ginseng using response surface methodology
摘要
Abstract
To optimize the extraction process for ginsenosides from the fibrous roots of American ginseng,the enzyme-water method was used for continuous extraction of ginsenosides from American ginseng.The total ginsenosides extraction yield was used as the evaluation index,and the extraction process was optimized using the response surface methodology.The results showed that the optimal enzymatic extraction conditions were as follows:α-amylase with an enzyme concentration of 7.3％,a liquid/solid ratio of 30 mL/g,and an enzymatic hydrolysis time of 1.2 h.After enzymatic hydrolysis,a secondary water extraction was performed,and the optimal conditions were as follows:a liquid/solid ratio of 27 mL/g,an extraction temperature of 89 ℃,and an extraction time of 1.3 h.Under these conditions,the total ginsenosides extraction yield reached 16.61％.Since it is stable,simple,and efficient,this method has significantly improved the extraction yield and avoided the waste of resources.It partially fills a gap in the extraction process of total ginsenosides from the fibrous roots of American ginseng and provides a reference for further purification,refinement,and activity studies of total ginsenosides.关键词
西洋参/须根/人参皂苷/提取工艺/响应面法/单因素试验/紫外分光光度法Key words
American ginseng/fibrous roots/ginsenosides/extraction process/response surface methodology/single factor experiment/ultraviolet spectrophotometry分类
医药卫生引用本文复制引用
王海燕,俞萍,王鑫,于红霞,周洁,方磊..响应面法优化酶-水连续提取西洋参须根人参皂苷[J].山东科学,2025,38(5):10-21,12.基金项目
"十四五"国家重点研发计划子课题(2023YFC3503802) （2023YFC3503802）
