山东科学2025，Vol.38Issue(5)：22-30,9.DOI:10.3976/j.issn.1002-4026.20240104
联合指纹图谱和活性综合评价的荆防颗粒质量控制研究
Quality control study of Jingfang granules through combined fingerprinting and comprehensive assessment of activity
摘要
Abstract
Combined fingerprinting and comprehensive assessment of activity techniques were used in this study to determine the quality control of Jingfang granules.Separation was performed using an Agilent TC-C18 chromatographic column(4.6 mm×250 mm,5 μm),gradient elution performed using an acetonitrile-0.8％formic acid aqueous solution,and ESI-TOF/mass spectrometer was used for analysis.The chemical constituents of Jingfang granules were identified through the precise molecular weight information of the parent and daughter ions of compounds and by consulting references.Molecular docking was used to examine the bonding ability of the following five chemical componentsto neuraminidase(NA):prim-O-glucosylcimifugin(POG),ferulic acid,hesperidin,naringin,and sec-O-glucosylhamaudol(SOGH).The antioxidant activity of the five components was evaluated by an ABTS free radical scavenging in vitro experiment.Fingerprinting and quantitative assay were used to evaluate the quality of Jingfang granules.From the results,twenty-four chemical components were identified within the Jingfang granules.Molecular docking results revealed the high binding capacity of NA to POG,ferulic acid,naringin,and SOGH,with a minimum recorded binding energy of＜-5 kcal/mol.Free radical scavenging in vitro experiments revealed that all the five components exhibited certain antioxidant activities.The compositional similarity of different batches of Jingfang granules was found to be relatively close,but there were some differences in the content of the five components,which may be attributed to differences in the sources of Chinese herbal medicines.This study provides methodological and conceptual support for overall quality evaluation studies of Jingfang granules.关键词
荆防颗粒/指纹图谱/活性综合评价/分子对接Key words
Jingfang granules/fingerprinting/comprehensive assessment of activity/molecular docking分类
医药卫生引用本文复制引用
苗冉,关永霞,郭焕滢,张贵民,王晓,赵恒强..联合指纹图谱和活性综合评价的荆防颗粒质量控制研究[J].山东科学,2025,38(5):22-30,9.基金项目
山东省重点研发计划(重大科技创新工程)(2021CXGC010508) （重大科技创新工程）
山东省泰山学者项目(tstp20221138) （tstp20221138）
财政部和农业农村部:国家现代农业产业技术体系(CARS-21) （CARS-21）
