山东科学2025，Vol.38Issue(5)：40-49,10.DOI:10.3976/j.issn.1002-4026.20240121
基于网络药理学和体外实验拓扑分析三七总皂苷治疗痛风性关节炎的作用机制
Topological analysis reveals mechanism of Panax notoginseng saponin in the treatment of gouty arthritis based on network pharmacology and in vitro experiments
摘要
Abstract
In this study,we analyzed the active components and mechanism of Panax notoginseng saponin(PNS)in treatment of gouty arthritis(GA)through network pharmacology,molecular docking,and cellular experiments.An in vitro hyperuricemia cell model was established using adenosine and xanthine oxidase to induce HK-2 cells.Moreover,the uric acid-lowering activity of PNS at different doses was assessed.Network pharmacology was used to explore the potential mechanism of PNS in the treatment of GA,and key targets were validated using molecular docking.Results revealed that PNS could inhibit the release of uric acid from renal tubular epithelial cells that was induced by adenosine and xanthine oxidase.In addition,15 key targets related to GA intervention were identified from PNS.Results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses suggested that PNS may exert therapeutic effects on GA by regulating multiple signaling pathways,such as JAK2-STAT3,AGE-RAGE,and calcium signaling.The molecular docking results showed that the binding energies of each active component with key targets such as STAT3,PTAFR,and IL2 were all lower than-5 kcal/mol,indicating good affinity,which can be used as potential therapeutic targets.This study provides a reference for the use of PNS in the treatment of GA.关键词
三七总皂苷/痛风性关节炎/网络药理学/分子对接/作用机制Key words
Panax notoginseng saponin/gouty arthritis/network pharmacology/molecular docking/mechanism分类
医药卫生引用本文复制引用
郭应建,于金倩,董红敬,王闯闯,钟恺,王晓..基于网络药理学和体外实验拓扑分析三七总皂苷治疗痛风性关节炎的作用机制[J].山东科学,2025,38(5):40-49,10.基金项目
国家重点研发计划(2023YFC3503805) （2023YFC3503805）
山东省泰山学者项目(tstp20221138) （tstp20221138）
济南市"新高校20条"(202228020) （202228020）
