山东农业科学2025，Vol.57Issue(9)：31-40,10.DOI:10.14083/j.issn.1001-4942.2025.09.004
稻瘟菌侵染对水稻氮素吸收利用途径相关基因表达的影响
Effects of Magnaporthe oryzae Infection on Expression of Genes Related to Nitrogen Absorption and Utilization Pathways in Rice
摘要
Abstract
Rice blast is the most devastating fungal disease in rice production.High nitrogen fertilizer ap-plication tends to promote rice susceptibility to blast,however,the underlying genetic basis remains unclear.To elucidate the role of nitrogen uptake and utilization pathways during Magnaporthe oryzae infection,in this study,the expression patterns of genes associated with rice nitrogen absorption,metabolism and utilization sig-naling pathways were analyzed by real-time RT-qPCR after blast inoculation.The results showed that among the 33 genes regulating nitrogen uptake and utilization,22 genes including 8 nitrate transporter genes,3 am-monium transporter genes,3 nitrogen assimilation-related genes,5 nitrogen signaling regulatory genes,and 3 nitrogen utilization-related genes were significantly up-regulated at 4 hours after infected by a compatible M.oryzaerace,which showed no significant changes compared to the control during the middle to late infection sta-ges(8～96 h);28 genes including 11 nitrate transporter genes,4 ammonium transporter genes,5 nitrogen as-similation-related genes,5 nitrogen signaling regulatory genes,and 3 nitrogen utilization-related genes were down-regulated upon inoculation with an incompatible M.oryzae race(4～96 h).These findings demonstrated that the activation of nitrogen absorption,metabolism,and utilization signaling pathways played critical roles in facilitating early M.oryzae infection,which could provide references for further unraveling the molecular mechanisms of nitrogen-induced susceptibility to rice blast.关键词
水稻/稻瘟病/氮素促进感病/氮素吸收利用信号途径/基因表达Key words
Rice/Rice blast/Nitrogen-induced susceptibility(NIS)/Nitrogen absorption and utiliza-tion signaling pathway/Gene expression分类
农业科技引用本文复制引用
黄浩,林晋军,陈文凤,姚威,陈倩茹,黄希来,吴俊,刘雄伦,刘金灵..稻瘟菌侵染对水稻氮素吸收利用途径相关基因表达的影响[J].山东农业科学,2025,57(9):31-40,10.基金项目
国家自然科学基金项目(31972256) （31972256）
湖南省2023年度十大技术攻关项目(2023NK1010) （2023NK1010）
