陕西中医2025，Vol.46Issue(10)：1305-1311,7.DOI:10.3969/j.issn.1000-7369.2025.10.002
固本平喘方对COPD模型大鼠NF-κB p65、IKBα、IL-1β mRNA表达的影响
Effects of Guben Pingchuan formula on the expression of NF-κB p65,IKBα,and IL-1β mRNA in a rat model of chronic obstructive pulmonary disease
摘要
Abstract
Objective:This study investigated the therapeutic potential and molecular mechanisms of Guben Pingchuan formula in COPD rats by analyzing NF-κB p65,IKBα,and IL-1β expression.Methods:Sixty-four SPF SD rats underwent 1-week acclimatization,with 52 subjected to COPD modeling via cigarette smoke exposure combined with intratracheal LPS and PPE instillation.Twelve untreated rats served as the blank group.Post-modeling,50 rats were randomized into five cohorts(n=10/group):model group,dexamethasone group(0.15 mg/kg),and the Guben Pingchuan formula High(28 g/kg),Medium(14 g/kg),and Low(7 g/kg)dosage groups.Ten blank group rats were randomly included.After 15 days,samples were collected post-euthanasia for:observing the histopathological changes in the lungs of rats by HE staining;to determine the level of the inflammatory factor IL-1β in the lung tis-sues by ELISA;western blot and immunofluorescence analysis of NF-κB p65 and IKBα protein expression.The rela-tive expression of IL-1β mRNA in lung tissue was sensed using RT-PCR.Results:The model group showed that se-vere alveolar and interstitial tissue were damaged with inflammatory cell infiltration,while dexamethasone and GPF groups exhibited attenuated pathology.Compared to the blank group,elevated IL-1β levels(P＜0.05)and NF-κB p65 expression(P＜0.05)accompanied by reduced IκBα(P＜0.05)were detected in other groups.Conversely,GPF and dexamethasone treatments significantly suppressed IL-1β,NF-κB p65,and IL-1β mRNA expression(P＜0.05),while upregulating IKBα(P＜0.05).Dose-dependent effects were evident:high-dose GPF showed stronger inhibition of in-flammatory markers and higher IKBα induction than low-dose(P＜0.05).Immunofluorescence results indicated that NF-κB p65 was strongly expressed in the model group and showed stronger fluorescence in the low-dose group of Guben Pingchuan formula.It was gradually weakened across Guben Pingchuan formula dose gradients.Conclusion:Guben Pingchuan formula can reduce NF-κB p65 protein expression and IL-1β content,increase IKBα protein expres-sion,and down-regulate IL-1β mRNA expression in the lung tissues of rats with COPD,thereby mitigating pulmona-ry inflammation and tissue damage.关键词
慢性阻塞性肺疾病/固本平喘方/大鼠/核因子-κB p65/炎症/人核因子κB抑制蛋白αKey words
Chronic obstructive pulmonary disease/Guben Pingchuan formula/Rats/Nuclear factor kappa-B p65 subunit/Inflammation/Inhibitor of nuclear factor kappa-B alpha分类
医药卫生引用本文复制引用
游慧娟,吴阳洋,欧阳桂兰,陈新海,游柏稳..固本平喘方对COPD模型大鼠NF-κB p65、IKBα、IL-1β mRNA表达的影响[J].陕西中医,2025,46(10):1305-1311,7.基金项目
湖南省中医药管理局重点项目(2021003) （2021003）
