食管疾病2025，Vol.7Issue(3)：173-181,9.DOI:10.15926/j.cnki.issn2096-7381.2025.03.003
单细胞测序分析食管鳞癌中癌相关成纤维细胞的分子特征
The Molecular Characteristics of Cancer-Associated Fibroblasts in Esophageal Squamous Cell Carcinoma by Single-Cell Sequencing Analysis
摘要
Abstract
Objective To establish a prognostic prediction model for Esophageal squamous cell carcinoma(ESCC)ESCC patients using characteristics of cancer-associated fibroblast(CAF)CAF obtained from single-cell sequencing analysis.Methods Single-cell sequencing data for ESCC,including 70 tumor and 8 normal tissue samples,were obtained from the datasets GSE160269,GSE196756,and GSE188900.We utilized the Findallmarkers function to identify all CAF's subpopulations for studying their roles in ESCC.Subsequently,the coxph function from the survival R package was employed to construct a proportional hazards model(Cox model).A prognostic model was then developed using this approach to predict patient survival rates.Results CAF exhibited a significant change in their proportions within ESCC tumor samples compared to normal samples.They were categorized into seven subgroups,each with distinct biological functions.We constructed a prognostic prediction model using the risk factors that are derived from CAF.This model can distinguish high-risk individuals from low-risk individuals.Conclusion This study unveils the molecular characteristics of CAF in ESCC and constructs a prognostic model with good predictive performance through the analysis of their characteristic genes.关键词
食管鳞状细胞癌/肿瘤微环境/癌相关成纤维细胞/差异表达基因/预后Key words
esophageal squamous cell carcinoma/tumor microenvironment/cancer-associated fibroblast/differentially expressed genes/prognosis分类
医药卫生引用本文复制引用
冯智坤,刘子玉,苏晟瑜,张源芳,薛经纬,江亚南..单细胞测序分析食管鳞癌中癌相关成纤维细胞的分子特征[J].食管疾病,2025,7(3):173-181,9.基金项目
河南省科技攻关项目(242102311082)河南省高等学校重点科研项目(23ZX007) （242102311082）
