水生生物学报2025，Vol.49Issue(11)：18-27,10.DOI:10.3724/1000-3207.2025.2025.0097
基于GBS技术的北方地区拉氏大吻鱥群体遗传多样性分析
GENETIC DIVERSITY ANALYSIS OF NORTHERN RHYNCHOCYPRIS LAGOWSKII POPULATIONS BASED ON GENOTYPING BY SEQUENCING TECHNOLOGY
摘要
Abstract
Rhynchochypris lagowskii is a small freshwater economic fish species selected by the Ministry of Agricul-ture and Rural Affairs as one of the top 10 characteristic aquatic germplasm resources in 2022.To investigate the genetic diversity of the R.lagowskii populations in northern China and provide references for its conservation and genetic breeding,Genotyping by sequencing(GBS)was used to develop single nucleotide polymorphism(SNP)mar-kers and analyze genetic characteristics of 109 individuals from four R.lagowskii populations in Ninghe,Jizhou,Juhe(Tianjin),and Taizihe(Liaoning).Through sequencing,a total of 3723530 SNP markers were obtained,and 133536 high-quality SNP loci were screened for genetic analysis after filtering.The expected heterozygosity(He)of the four populations was 0.0397-0.0884,the observed heterozygosity(Ho)was 0.0394-0.0818,the nucleotide diversity(Pi)was 0.0406-0.098,the polymorphic information content(PIC)value was 0.0327-0.0717,the genetic differentiation coefficient(FST)was 0.0039-0.8148,and the inbreeding coefficient(Fis)within population was 0.0567-0.5480.Genetic structure analysis showed that four populations clustered into three distinct groups,with individuals from the Ninghe and Jizhou populations in Tianjin clustered into one branch,and individuals from the Taizihe and Juhe popula-tions in Liaoning and Tianjin clustered into one branch,respectively.Population specific SNP markers were identified for all four populations,serving as candidate molecular markers for species identification,population genetics,trait association analysis,and evolutionary studies.Population history dynamics research indicates that the four populations of R.lagowskii all underwent a process characterized by a sharp decline in effective population size(Ne)during the Quaternary glacial period.关键词
GBS/遗传多样性/SNP标记/遗传结构/种群动态/拉氏大吻鱥Key words
GBS/Genetic diversity/SNP marker/Genetic structure/Population dynamics/Rhynchocypris lagowskii分类
生物科学引用本文复制引用
白晓慧,刘克明,何晓旭,尤宏争,骆小年,汪笑宇,薄其康,张韦..基于GBS技术的北方地区拉氏大吻鱥群体遗传多样性分析[J].水生生物学报,2025,49(11):18-27,10.基金项目
天津市科技计划(22ZYCGSN00820)资助[Supported by the Tianjin Science and Technology Plan Project(22ZYCGSN00820)] （22ZYCGSN00820）
