水生生物学报2025，Vol.49Issue(11)：28-39,12.DOI:10.3724/1000-3207.2025.2025.0152
长江流域中华沙塘鳢与河川沙塘鳢基于线粒体Cyt b基因的种群遗传及形态分化研究
POPULATION GENETIC AND MORPHOLOGICAL DIFFERENCES OF ODON-TOBUTIS SINENSIS AND O.POTAMOPHILUS IN YANGYZE RIVER BASIN BASED ON MITOCHONDRIAL CYT B GENE
摘要
Abstract
In order to clarify the differences between the Odontobutis sinensis and O.potamophilus,population gene-tics were studied based on Cyt b gene and morphological characteristics were investigated using traditional morphologi-cal character measurement and truss method.21 haplotypes were identified for 128 individuals of O.sinensis from 3 locations,and 28 haplotypes for 195 individuals of O.potamophilus from 4 locations.Haplotype diversity and nucleotide diversity of O.sinensis were 0.868 and 0.00407,respectively,while those of O.potamophilus were 0.910 and 0.02000.Phylogenetic analyses for all haplotypes based on BI and ML methods revealed that all Odontobutis species form a monophyletic group.Results showed that O.sinensis has 2 lineages and O.potamophilus consists of 3 lineages,with an obvious pattern of geographic distribution.AMOVA results indicated that the genetic differentiation(FST)among geographical populations of O.sinensis ranged from 0.463-0.845,while for O.potamophilus,it ranged from 0.653-0.930,suggesting significant genetic differentiation among populations.This finding implies that the most probable geographic differentiation of the populations.Mismatch distribution and neutrality test revealed that XN popu-lation of O.sinensis have experienced the population expansion event,and it may happen at 0.20 Ma.Slight diffe-rences in the results of historical dynamic analyses across methods suggest a relatively complex demographic history of Odontobutis.Morphological analysis revealed distinct variations in O.sinensis and O.potamophilus.Principal compo-nents analysis showed that most individuals of two species were separated.Then the discrimination functions of O.sinensis and O.potamophilus were set up by stepwise discriminant analysis with an accuracy rate of 95.7%(P<0.01).The eight components with the highest contributions to principal components analysis and discriminant analysis were related to the characteristics of fin and trunk.This quantitative divergence(QST)was not significantly correlated with the genetic differentiation(FST),and the degree of FST was significantly high than that of QST,which suggested that phenotypes were favored and stable selection occurred in different populations.关键词
形态分化/Cyt b/遗传多样性/主成分分析/判别分析/中华沙塘鳢/河川沙塘鳢Key words
Morphological difference/Cyt b/Genetic diversity/Principal component analysis/Discriminant analysis/Odontobutis sinensis/Odontobutis potamophilus分类
生物科学引用本文复制引用
王雪,刘怡然,任艳,谢德兵,周莉,李波,王守荣,朱思华..长江流域中华沙塘鳢与河川沙塘鳢基于线粒体Cyt b基因的种群遗传及形态分化研究[J].水生生物学报,2025,49(11):28-39,12.基金项目
武汉市农科院2024年创新体系学科创新项目(XKCX202403-3) （XKCX202403-3）
武汉市科技特派员"产学研"专项项目(2023110201030683)资助[Supported by the 2024 Wuhan Academy of Agricultural Sciences Science and Technology Innovation Project(XKCX202403-3) （2023110201030683）
the Special Program for Wuhan Science and Technology Commissioner"Industry-University-Research"(2023110201030683)] （2023110201030683）
