水生生物学报2025，Vol.49Issue(11)：82-93,12.DOI:10.3724/1000-3207.2025.2025.0009
低鱼粉饲料中添加丁酸钠对黄颡鱼生长性能、血清免疫及肠道健康的影响
SODIUM BUTYRATE ADDITION TO LOW FISHMEAL DIETS ON GROWTH PERFORMANCE,SERUM IMMUNITY,AND INTESTINAL HEALTH OF CATFISH PELTEOBAGRUS FULVIDRACO
摘要
Abstract
The experiment was designed to investigate the effects of sodium butyrate addition at different concentra-tions to the feed on growth performance,serum biochemical indices,intestinal antioxidant,and digestive enzymes in Pelteobagrus fulvidraco.A high-fishmeal diet(20%fishmeal and 0 cottonseed protein;positive control)and a low-fish-meal diet(10%fishmeal,18.4%cottonseed protein;negative control)were formulated.Sodium butyrate was added to the low fishmeal diet group at 0.1%and 0.15%,respectively,resulting in four isonitrogenous and isofat experimental feeds by adjusting chicken meal,pork meal,maize protein meal,and soya bean meal.Pelteobagrus fulvidraco with an initial weight of 11 g was selected and randomly divided into four groups with three replicates(40 fish/replicate)and fed diets for a period of 56d.The results showed that compared with the high fishmeal group,the average final weight,weight gain rate,alkaline phosphatase(AKP),lipase(LPS),superoxide dismutase(SOD)activity,expression of the anti-inflammatory factor IL-10,and the length of intestinal villi,as well as the contents of Pseudomonas spp.and Methylobacillus spp.of Pelteobagrus fulvidraco in the low-fishmeal group were all significantly decreased(P<0.05),whereas serum ghrelin transaminase(GPT),ghrelintransaminase(GOT),and intestinal malondialdehyde(MDA)levels were significantly increased(P<0.05),the expression of intestinal pro-inflammatory factor TNF-α,pro-apoptotic genes P53,and Caspase-3 was significantly up-regulated(P<0.05),and the level of Pseudomonas spp.and Methylobacillus spp.in the intestines was significantly increased(P<0.05).After the addition of sodium butyrate to the feed with low fishmeal,the final mean weight as well as the weight gain rate showed a significant increase(P<0.05)compared to the low fishmeal group.The serum glutamate transaminase(GPT)and glutamate oxaloacetate transaminase(GOT)levels of Pelteobagrus fulvidraco in the group with 0.10%sodium butyrate were significantly decreased(P<0.05),whereas the serum levels of alkaline phosphatase(AKP),acid phosphatase(ACP),and urea nitrogen(BUN)levels were signifi-cantly increased(P<0.05),LPS,AMS,and SOD levels were significantly elevated(P<0.05)and MDA levels were significantly decreased(P<0.05)in the group supplemented with sodium butyrate,and peroxidase(CAT)content was significantly elevated(P<0.05)in the group supplemented with 0.15%sodium butyrate.The expression of TNF-α,P53,and Caspase-3 was down-regulated(P<0.05),the expression of IL-10 and Occludin was significantly up-regulated(P<0.05),the height of intestinal villi was significantly increased(P<0.05)in the group of added sodium butyrate,and the level of Pseudomonas spp.in the intestines was significantly reduced(P<0.05)in the group of 0.15%sodium butyrate.In conclusion,dietary sodium butyrate supplementation improve growth performance,enhance intestinal antioxidant capacity,alleviate intestinal inflammation,promote villi development,and suppress harmful flora micro-biota in Pelteobagrus fulvidraco,thereby maintaining the homeostasis of the intestinal flora.关键词
丁酸钠/生长/肠道/抗氧化/菌群/黄颡鱼Key words
Sodium butyrate/Growth/Intestine/Antioxidant/Bacterial flora/Pelteobagrus fulvidraco分类
农业科技引用本文复制引用
艾业睿,胡毅,张俊智,蔡明浪,王绍,常翠维,谢永宏,钟蕾..低鱼粉饲料中添加丁酸钠对黄颡鱼生长性能、血清免疫及肠道健康的影响[J].水生生物学报,2025,49(11):82-93,12.基金项目
湖南省揭榜挂帅项目:洞庭湖总磷污染防治关键技术研究与示范(2024QK3001)资助[Supported by the Research and Demon-stration of Key Technologies for the Prevention and Control of Total Phosphorus Pollution in Dongting Lake(2024QK3001)] （2024QK3001）
