陕西医学杂志2025，Vol.54Issue(10)：1299-1304,6.DOI:10.3969/j.issn.1000-7377.2025.10.001
吴茱萸碱调节音猬因子/家族锌指蛋白1通路对免疫球蛋白A肾病大鼠肾损伤的影响
Effect of evodiamine on renal damage in IgA nephropathy rats by modulating Shh/Gli1 pathway
摘要
Abstract
Objective:To explore the effect of evodiamine(Evo)on renal damage in immunoglobulin A nephrop-athy(IgAN)rats by modulating sonic hedgehog(Shh)/Glioma-associated oncogene homolog 1(Gli1)pathway.Methods:SD rats were stochastically assigned into NC group,IgAN group,L-Evo group(20 mg/kg Evo),H-Evo group(40 mg/kg Evo),and H-Evo+PUR group(40 mg/kg Evo+5 mg/kg Shh activator Purmorphoamine).Fully automated biochemical analyzer was used to measure urine 24-hour urine protein,urine red blood cells,and serum creatinine,blood urea nitrogen,and albumin in each group.ELISA was used to measure serum tumor necrosis factor α (TNF-α)and interleukin 1β(IL-1β)in each group,and HE staining was used to observe the pathological morphology of renal tissue in each group.Immunofluorescence staining was used to observe IgA and α-SMA in renal tissues of each group.Western blot was used to detect Shh,Gli1,and extracellular matrix deposition related proteins in kidney tissues of each group.Results:For the NC group,the IgAN group showed an increase in 24-hour urine protein,urine red blood cells,and serum creatinine,blood urea nitrogen,TNF-α,and IL-1β,severe renal tissue pathological damage,an increase in IgA,α-SMA,Shh,Gli1,Fibronectin,Collagen,and Laminin in renal tissue,and a decrease in serum al-bumin(all P＜0.05).For the IgAN group,the L-Evo group and H-Evo group showed a decrease in 24-hour urine protein,urine red blood cells,and serum creatinine,blood urea nitrogen,TNF-α,and IL-1β,reduced renal tissue patho-logical damage,a reduction in IgA,α-SMA,Shh,Gli1,Fibronectin,Collagen,and Laminin in renal tissue,and a raise in serum albumin(all P＜0.05).For the H-Evo group,the H-Evo+PUR group showed an increase in 24-hour urine protein,urine red blood cells,and serum creatinine,blood urea nitrogen,TNF-α,and IL-1β,severe renal tissue patho-logical damage,an increase in IgA,α-SMA,Shh,Gli1,Fibronectin,Collagen,and Laminin in renal tissue,and a de-crease in serum albumin(all P＜0.05).Conclusion:Evo may inhibit Shh/Gli1 pathway,suppress inflammatory re-sponses,reduce IgA,α-SMA,and extracellular matrix deposition,hinder the process of renal fibrosis,and improve pathological damage to renal tissue.关键词
吴茱萸碱/免疫球蛋白A肾病/音猬因子/Gli家族锌指蛋白1/肾损伤/作用机制Key words
Evodiamine/Immunoglobulin A nephropathy/Sonic hedgehog/Glioma-associated oncogene homo-log 1/Renal damage/Mechanism of action分类
医药卫生引用本文复制引用
任永昊,王一川..吴茱萸碱调节音猬因子/家族锌指蛋白1通路对免疫球蛋白A肾病大鼠肾损伤的影响[J].陕西医学杂志,2025,54(10):1299-1304,6.基金项目
国家自然科学基金资助项目(82204881) （82204881）
