陕西医学杂志2025，Vol.54Issue(10)：1311-1315,1348,6.DOI:10.3969/j.issn.1000-7377.2025.10.003
山奈酚调节Hippo信号通路对脊髓星形胶质细胞氧化损伤的影响
The effect of kaempferol on oxidative damage of spinal cord astrocytes by regulating the Hippo signaling pathway
摘要
Abstract
Objective:To explore the effect of kaempferol on oxidative damage of spinal cord astrocytes by regu-lating the Hippo signaling pathway.Methods:Rat spinal cord astrocytes were assigned into control group,oxidative damage group,L-kaempferol group,H-kaempferol group,and H-kaempferol+inhibitor group.Except for the control group,all other groups were cultured with 200 μmol/L H2 O2 for 24 hours to induce oxidative damage.MTT assay was performed to detect cell viability.TUNEL staining was used to detect apoptosis.The reagent kits were used to detect the malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px).Reactive oxygen species fluorescent probe was used to measure reactive oxygen species.Western blot was performed to detect the Yes associated protein 1(YAP)protein in spinal cord astrocytes.Results:Compared with the control group,the oxidative damage group showed a decrease in cell viability,an increase in TUNEL positive cell rate,a decrease in GSH-Px and SOD,an increase in MDA and reactive oxygen species,and a decrease in p-YAP protein(all P＜0.05).Compared with the oxidative damage group,the L-kaempferol group and H-kaempferol group showed increased cell viability,decreased TUNEL positive cell rate,increased GSH-Px and SOD,decreased MDA and reactive oxygen spe-cies,and increased p-YAP protein(all P＜0.05),the changes in spinal cord astrocytes in the H-kaempferol group were greater than those in the L-kaempferol group(P＜0.05).YAP inhibitor inhibited the p-YAP protein in spinal astrocytes,exacerbated oxidative damage in spinal astrocytes(P＜0.05).Conclusion:Kaempferol regulates the Hippo signaling pathway to alleviate oxidative damage in spinal cord astrocytes.关键词
山奈酚/Hippo信号通路/脊髓星形胶质细胞/氧化损伤/脊髓损伤Key words
Kaempferol/Hippo signaling pathway/Spinal cord astrocytes/Oxidative damage/Spinal cord injury分类
医药卫生引用本文复制引用
张君,崔益华,黄成,卢弘栩,冯小燕..山奈酚调节Hippo信号通路对脊髓星形胶质细胞氧化损伤的影响[J].陕西医学杂志,2025,54(10):1311-1315,1348,6.基金项目
国家自然科学基金资助项目(32071128) （32071128）
江苏省南通市社会民生科技计划项目(MSZ2024154) （MSZ2024154）
