陕西医学杂志2025，Vol.54Issue(10)：1331-1336,6.DOI:10.3969/j.issn.1000-7377.2025.10.006
基于Janus激酶2-信号转导器和转录激活因子3-孤独核受体通路探讨人参皂苷Rg1对慢性阻塞性肺疾病模型肺功能的影响
Effects of ginsenoside Rg1 on lung function in a chronic obstructive pulmonary disease model via the JAK2-STAT3-RORγt pathway
摘要
Abstract
Objective:To investigate the effects of ginsenoside Rg1 on lung function in a chronic obstructive pul-monary disease(COPD)model via the JAK2-STAT3-RORγt pathway.Methods:Fifty 4-to 5-week-old rats were randomly divided into 5 groups:control group,model group,low-dose ginsenoside Rg1 group(5 mg/kg),high-dose ginsenoside Rg1 group(20 mg/kg),and high-dose ginsenoside Rg1+CA1 group(20 mg/kg+4 mg/kg),with 10 rats in each group.A rat model of COPD was established by cigarette smoke exposure Lung function was assessed using a pulmonary function tester to measure forced vital capacity(FVC),forced expiratory volume at 0.1 seconds(FEV0.1),and peak expiratory flow(PEF).Arterial blood gas analysis was performed to determine partial pressure of oxygen(PaO2),oxygen saturation(SaO2),and partial pressure of carbon dioxide(PaCO2).Enzyme-linked immu-nosorbent assay(ELISA)was used to detect levels of inflammatory cytokines IL-6,IL-17 and TNF-α in bronchoalve-olar lavage fluid.Lung tissue morphology was observed by HE staining.Western blot was employed to analyze the protein expression levels of JAK2,STAT3,p-STAT3 and RORγt in lung tissues.Results:Compared with the control group,the model group exhibited severe lung tissue damage,decreased FVC,FEV0.1,PEF,PaO2,and SaO2 levels(all P＜0.05),and increased PaCO2,IL-6,IL-17,TNF-α levels,and protein expression of JAK2,STAT3,p-STAT3.and RORγt(all P＜0.05).In contrast,the high-dose ginsenoside Rg1 groups showed alleviated lung tissue injury,in-creased FVC,FEV0.1,PEF,PaO2,and SaO2 levels(all P＜0.05),and decreased PaCO2,IL-6,IL-17,TNF-α levels,and JAK2,STAT3,p-STAT3,and RORγt protein expression(all P＜0.05)compared with the model group.The high-dose ginsenoside Rg1 group demonstrated more pronounced improvements in lung function and greater reductions in in-flammatory parameters and pathway activation than the low-dose group(all P＜0.05).Compared with the high-dose ginsen-oside Rg1 group,the high-dose ginsenoside Rg1+CA1 group showed exacerbated lung tissue damage,reduced lung function indices,increased inflammatory cytokine levels,and upregulated JAK2-STAT 3-RORγt pathway activation(all P＜0.05).Conclusion:Ginsenoside Rg1 improves lung function in COPD model rats by reducing inflammatory cytokine levels,and its mechanism may be associated with the inhibition of the JAK2-STAT 3-RORγt signaling pathway.关键词
慢性阻塞性肺疾病/人参皂苷Rg1/炎症/Janus激酶2/信号转导与转录激活因子3/孤独核受体Key words
Chronic obstructive pulmonary disease/Ginsenoside rg1/Inflammation/JAK2/STAT3/RORγt分类
医药卫生引用本文复制引用
杨媛,朱佳..基于Janus激酶2-信号转导器和转录激活因子3-孤独核受体通路探讨人参皂苷Rg1对慢性阻塞性肺疾病模型肺功能的影响[J].陕西医学杂志,2025,54(10):1331-1336,6.基金项目
新疆维吾尔自治区卫生健康保健科研专项课题(BL202404) （BL202404）
