沈阳医学院学报2025，Vol.27Issue(5)：467-476,10.DOI:10.16753/j.cnki.1008-2344.2025.05.004
AHNAK核蛋白2在甲状腺癌中的表达及作用机制
Expression and mechanism of AHNAK2 in thyroid cancer
摘要
Abstract
Objective:To investigate the expression and biological function of AHNAK nucleoprotein 2(AHNAK2)in thyroid cancer.Methods:The expression of AHNAK2 in thyroid cancer tissues was analyzed using The Cancer Genome Atlas(TCGA),Gene Expression Omnibus(GEO),and Human Protein Atlas(HPA)databases.The correlation between AHNAK2 expression and patients'prognosis,clinicopathological characteristics,immune cell infiltration,and subcellular localization was evaluated.AHNAK2 mRNA expression in thyroid cancer cells was detected by RT-qPCR.A stable AHNAK2 knockdown TPC-1 cell line was established via lentiviral transfection,and the transfection efficiency was validated using RT-qPCR and Western blot.TPC-1 cells were divided into the sh-NC and sh-AHNAK2 groups.The impact of AHNAK2 knockdown on TPC-1 cell proliferation,migration,and invasion was assessed using colony formation assay,CCK-8 assay,wound healing assay,and Transwell assay.Results:AHNAK2 was up-regulated in thyroid cancer tissues and was associated with poor prognosis.It was primarily localized in the cytoplasm and cytoplasmic membrane.GO analysis revealed that AHNAK2 was mainly enriched in biological processes such as organelle disassembly,cellular components such as the spindle,and molecular functions such as microtubule binding.KEGG pathway analysis indicated that AHNAK2 was primarily enriched in the cell cycle pathway.Immune infiltration analysis suggested that AHNAK2 expression was positively correlated with macrophage infiltration and negatively correlated with plasmacytoid dendritic cell infiltration in thyroid cancer.AHNAK2 was overexpressed in multiple thyroid cancer cell lines.In vitro experiments demonstrated that AHNAK2 knockdown inhibited the proliferation,migration,and invasion of TPC-1 cells.Conclusion:AHNAK2 may serve as an important prognostic biomarker and immunotherapy target for thyroid cancer,as it can promote the proliferation,migration and invasion of thyroid cancer cells.关键词
甲状腺癌/AHNAK核蛋白2/免疫浸润/细胞迁移/细胞侵袭Key words
thyroid cancer/AHNAK2/immune infiltration/cell migration/cell invasion分类
医药卫生引用本文复制引用
陈晶晶,俞建华,张凤霞,孙成林..AHNAK核蛋白2在甲状腺癌中的表达及作用机制[J].沈阳医学院学报,2025,27(5):467-476,10.基金项目
辽宁省科学技术计划重大科研项目(No.2022JH2/101300035) （No.2022JH2/101300035）
沈阳市科学技术计划项目(No.21-173-9-18) （No.21-173-9-18）
