外科理论与实践2025，Vol.30Issue(4)：295-301,7.DOI:10.16139/j.1007-9610.2025.04.02
miR-4674表达变化对BGC-823胃癌细胞系生物学特征的影响
Impact of miR-4674 expression changes on the biological characteristics of BGC-823 gastric cancer cell line
摘要
Abstract
Objective To explore the impact of miR-4674 expression changes on the biological characteristics of BGC-823 gastric cancer cell line based on bioinformatics research.Methods Through bioinformatics screening,miR-4674 was prioritized as a gastric cancer-associated miRNA.We constructed miR-4674 mimic,inhibitor,and corresponding negative control(NC)transfected into the BGC-823 cell line.Reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect the expression changes of miR-4674 in BGC-823 cells.Functional assays included:MTT assay for cell proliferation ability;Transwell assay for migration capacity;TUNEL staining for cell apoptosis detection.Results Compared with the control group and cells transfected with NC,the level of miR-4674 was significantly increased in cells transfected with miR-4674 mimic,and the proliferation and migration abilities of the cells were significantly improved(P<0.05).In cells transfected with miR-4674 inhibitor,the level of miR-4674 was significantly decreased,and the proliferation and migration abilities of the cells were significantly reduced(P<0.05).The result of TUNEL showed no significant differences in apoptotic rates were observed across all groups.Conclusions In the BGC-823 gastric cancer cell line,inhibition of miR-4674 expression can reduce its malignancy,while overexpression of miR-4674 can enhance its malignancy,suggesting its potential as a therapeutic target for gastric cancer intervention,providing a new strategy for the treatment of gastric cancer.关键词
miR-4674/胃癌/BGC-823细胞系/恶性程度Key words
miR-4674/Gastric cancer/BGC-823 cell line/Malignancy degree分类
医药卫生引用本文复制引用
袁小兵,朱建伟..miR-4674表达变化对BGC-823胃癌细胞系生物学特征的影响[J].外科理论与实践,2025,30(4):295-301,7.基金项目
如皋市指导性科技攻关计划项目[SRGS(23)009] （23）
