西北农业学报2025，Vol.34Issue(10)：1822-1833,12.DOI:10.7606/j.issn.1004-1389.2025.10.006
蚯蚓粪与微生物菌剂配施对民勤蜜瓜生长及土壤微环境的影响
Effects of Vermicompost Combined with Microbial Agents on Growth of Minqin Cucumis melo and Soil Microenvironment
摘要
Abstract
To address the critical issues such as soil organic matter deficiency,frequent soil-borne dis-eases caused by long-term monocropping,and inconsistent fruit quality in the Minqin Cucumis melo cultivation area,this study employed vermicompost and microbial agents as effective,economical,and environmentally friendly soil amendments.The study investigated the synergistic effects of ver-micompost and microbial agents on Cucumis melo growth,soil enzyme activity,and microbial diversi-ty.The results showed that the combined application of vermicompost and microbial agents signifi-cantly increased Cucumis melo yield,vitamin C content,and sugar content,while reducing fruit hard-ness.The highest yield was observed with the treatment of triple-dose vermicompost combined with rhizosphere-promoting bacteria(RPB),which increased yield by 16.9％compared to the treatment with half-dose vermicompost and RPB.The activities of α-1,4-glucosidase and polyphenol oxidase in-creased with higher vermicompost dosages,while the activities of peroxidase and alkaline phosphatase decreased at the phylum level,the application of vermicompost and microbial agents increased the rel-ative abundance of Gemmatimonadetes and Actinobacteria,while reduced the relative abundance of Bacteroidetes.Based on the Chao1 index and Observed features index,the treatment with double dose of earthworm cast and EM microbial agent in the 20-40 cm soil layer exhibited the highest number of OTUs;while according to the Shannon index and Simpson index.The treatment with double-dose ver-micompost and EM microbial agent had the highest number of OTUs at the 20-40 cm depth of soil.Microbial agent applications shifted the soil bacterial community structure and stabilized the bacterial symbiotic networks.Overall,the triple-dose vermicompost combined with RPB is recommended as the optimal soil amendment strategy for Cucumis melo cultivation in Minqin region.关键词
蚯蚓粪/微生物菌剂/酶活性/细菌群落结构/蜜瓜品质Key words
Earthworm manure/Microbial agents/Enzyme activity/Bacterial community structure/Cucumis melo quality引用本文复制引用
马凤捷,杨春胤,王振龙,张靖,施志国..蚯蚓粪与微生物菌剂配施对民勤蜜瓜生长及土壤微环境的影响[J].西北农业学报,2025,34(10):1822-1833,12.基金项目
甘肃省青年科技基金计划(22JR5RA791,22JR11RA280) （22JR5RA791,22JR11RA280）
甘肃省软科学基金(23JRZH346) （23JRZH346）
中央引导地方科技发展资金项目(23ZYQH0298). Gansu Provincial Youth Science and Technology Foundation(No.22JR5RA791,No.22JR11RA280) （23ZYQH0298）
Gansu Provincial Soft Science Foundation(No.23JRZH346) （No.23JRZH346）
the Central Govern-ment Guides Local Science and Technology Development Fund Projects(No.23ZYQH0298). （No.23ZYQH0298）
