基于甘芪纯系群体高抗麻口病特性关联的蛋白质的筛选
Screening of Proteins Associated with High Resistance to Makou Disease in Ganqi Pure Line Population
摘要
Abstract
This study used intensity-based absolute quantification(iBAQ),a label-free quantitative proteomics technique,to analyze protein expression differences between Ganqi pure line strains(HQ1,HQ6,HQ19)exhibiting high resistance to Makou disease and a susceptible Astragalus control(CK).The aim was to elucidate the disease-resistance mechanism of Ganqi lines at the proteomic level.Total proteins were extracted from HQ1,HQ6,HQ19,and CK,separated via SDS-polyacrylamide gel elec-trophoresis(SDS-PAGE),digested in-gel,and analyzed using liquid chromatography-tandem mass spectrometry(LC-MS/MS).Resulting spectra were searched against the Uniprot database,and iBAQ was used for quantitative analysis.Differentially expressed and specifically expressed proteins were identified and subjected to Gene Ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.A total of 169 proteins were identified,eight of which exhibited statistically significant differential expression(P＜0.05)between Ganqi strains and CK.Six proteins were uniquely expressed in Ganqi strains.Notably,NADH dehydrogenase subunit 7 and sucrose synthase were identified among both differentially and specifically expressed proteins.GO analysis revealed that most differentially expressed proteins were enriched in molecular functions related to ATP synthesis.Specifically expressed proteins were predominantly associated with biological processes such as the biosynthesis of cinnamic acid and ergosterol,as well as the catabolism of sucrose,acetyl-CoA,and L-phenylalanine.KEGG enrichment showed that approximately 50％of these proteins participated in key metabolic pathways,including energy metabolism,amino acid metab-olism,and lipid metabolism.This study successfully identified multiple proteins associated with high resistance to Makou disease in Ganqi strains,including NADH dehydrogenase subunit 7,sucrose syn-thase,chalcone synthase,and non-specific serine/threonine protein kinases.These findings provide a foundation for analyzing the mechanism and causes of Astragalus's resistance to Makou disease.关键词
甘芪纯系群体/麻口病/蛋白质组学/差异表达蛋白/SDS-聚丙烯酰氨凝胶电泳Key words
Ganqi pure line population/Makou disease/Proteomics/Differentially expressed pro-teins/SDS-PAGE引用本文复制引用
吴永莉,郭苏杭,何春雨,张蕊菊,周珊,张延红,郭清毅..基于甘芪纯系群体高抗麻口病特性关联的蛋白质的筛选[J].西北农业学报,2025,34(10):1856-1866,11.基金项目
甘肃省高等学校青年博士基金(2021QB-073) （2021QB-073）
引进人才科研启动基金(2019YJRC-06) （2019YJRC-06）
民生科技专项(20CX9NA070) （20CX9NA070）
中央引导地方科技发展专项(22ZY1QA003/2025ZY036/YDZX20206200002673) （22ZY1QA003/2025ZY036/YDZX20206200002673）
甘肃省科技计划(重点研发计划)(24YANA007/23YFFA0027/24CXKA009/22CX8NA082/25JRRA1178) （重点研发计划）
高校产业支撑计划(2024CYZC-40/2020C-09). Youth Doctoral Fund of Higher Education Institutions in Gansu Province(No.2021QB-073) （2024CYZC-40/2020C-09）
Talent Introduction and Research Start up Fund(No.2019YJRC-06) （No.2019YJRC-06）
Special Project of Livelihood Science and Technology(No.20CX9NA070) （No.20CX9NA070）
Special Project for Local Science and Tech-nology Development Guided by the Central Government(No.22ZY1QA003/2025ZY036/YDZX20206200002673) （No.22ZY1QA003/2025ZY036/YDZX20206200002673）
Science and Technology Plan of Gansu Province(No.24YANA007/23YFFA0027/24CXKA009/22CX8NA082/25JRRA1178) （No.24YANA007/23YFFA0027/24CXKA009/22CX8NA082/25JRRA1178）
University Industry Support Program(No.2024 CYZC-40/2020C-09). （No.2024 CYZC-40/2020C-09）
