刑事技术2025，Vol.50Issue(5)：504-509,6.DOI:10.16467/j.1008-3650.2024.0090
一种罂粟源性实时荧光定量PCR检测方法的建立
Establishment of a Real-time Fluorescence Quantitative PCR Detection Method for Poppy Source
摘要
Abstract
In this study,poppy was used as the research object to construct a TaqMan probe real-time fluorescence quantitative PCR detection system for rapid and accurate detection of poppy origin,and to verify its application in actual samples.According to the ITS gene sequence of Papaver somniferum published in GenBank,the specific primers and probes were designed,and the amplification system was constructed and optimized.The specificity,sensitivity and the detection performance on actual samples were compared with the Sanger sequencing method to evaluate its feasibility.The results showed that the optimized real-time fluorescence quantitative PCR detection system had strong specificity,and the detection limit was 0.4 ng/μL.The poppy-derived components were detected in 18 samples to be tested,which were consistent with the results of DNA sequencing.The establishment of this research method has certain application potential for rapid detection of illegal cultivation of opium poppy-derived plants.关键词
植物种属鉴定/检测方法/荧光定量PCR/罂粟/ITS基因Key words
identification of plant species/detection method/fluorescence quantitative PCR/Papaver/ITS gene分类
社会科学引用本文复制引用
唐立冈,谭晴晴,杨雪,王文燕,孙庆东,张全芳..一种罂粟源性实时荧光定量PCR检测方法的建立[J].刑事技术,2025,50(5):504-509,6.基金项目
超强检验鉴定关键技术与成套装备研发(2021CXGC011305) （2021CXGC011305）
山东省创新公共服务平台计划-中药分子鉴定公共服务平台(2018JGX111) （2018JGX111）
山东省高校中药质量控制与全产业链建设协同创新中心(CYLXTCX2020) （CYLXTCX2020）
山东省农业科学院"3237"齐鲁农科英才工程(创新类) （创新类）
